地塞米松对急性肺损伤中TREM-1表达的影响

doi:10.13418/j.issn.1001-165x.2016.05.010

Chinese Journal Of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (5): 523-527.doi: 10.13418/j.issn.1001-165x.2016.05.010

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Effect of dexamethasone on the expression of TREM-1 in acute lung injury

LUO Feng¹, ZHENG Hao-xiang², DONG Liang³, LI Nian-wang², ZHOU Qin², JIA Zhe², HE Li-ping¹, SUN Guo-ying ^1,4

1.Department of Histology and Embryology, Hunan Normal University, Changsha 410013; 2. Clinical Medical Undergraduates of Grade 2014, College of Shu Da, Hunan Normal University, Changsha 410013; 3.Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563000;4. College of Basic Medical Sciences, Central South University, Changsha 410006, China

Received:2016-04-21 Online:2016-09-25 Published:2016-10-14

Abstract

Abstract:

Objective　To investigate the effects of dexamethasone (Dex) on the TREM-1expression in acute lung injury(ALI) of mice induced by lipopolysaccharide (LPS).　Methods Kunming mice (KM mice) were used as the research object and LPS (10 mg/kg) was used to induce acute lung injury model. After half an hour given different concentrations of Dex (5, 10, 20, 40 mg/kg) and selection of different time points (6, 12, 24 and 36 h) were observed. The lung tissue was observed by HE staining, RT-PCR was used to detect TREM-1 expression, and ELISA was used to detect the expression of sTREM-1 in bronchoalveolar lavage fluid of mice. Results Dex could time-dependently down-regulate the expression of lung tissue in the acute lung injury and in 6h treatment peak; Dexcould dose-dependently down-regulate TREM-1 expression in acute lung injury of lung tissue, and reached the peak at 10 mg /kg. Dex could decrease the protein level of sTREM-1 in bronchoalveolar lavage fluid of ALI mice.　Conclusion　Dex can down-regulate TREM-1 of lung tissues of ALI mice in a dose and time-dependent manner, and reduce myeloid cell membrane of TREM-1 in lung. Our research indicates that Dex may inhibit inflammatory cascade and protect lung tissue by regulation of TREM-1 expression.

Key words: Triggering receptor expressed on myeloid cells-1, Acute lung injury, Dexamethasone;Lipopolysaccharide

LUO Feng, ZHENG Hao-xiang, DONG Liang, LI Nian-wang, ZHOU Qin, JIA Zhe, HE Li-ping,SUN Guo-ying. Effect of dexamethasone on the expression of TREM-1 in acute lung injury[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 523-527.

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Effect of dexamethasone on the expression of TREM-1 in acute lung injury

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