电针刺激联合BMSCs移植对脑出血大鼠出血灶周边区水通道蛋白4表达的影响

doi:10.13418/j.issn.1001-165x.2016.06.012

Abstract

Abstract:

Objective　To explore effects of electro-acupuncture stimulation combined with bone marrow mesenchymal stem cells( BMSCs) transplantation on the expression of Aquaporins-4（AQP-4）in rats after intracerebral hemorrhage.　Methods　ICH rats models were established by injecting the collagenase and heparin into the right caudate nucleus. After 3 days, the animals were treated with BMSCs transplantation and electro-acupuncture (Ea-BMSCs group), or dealt with BMSCs transplantation (BMSCs group), electro-acupuncture (Ea group) and physiological saline injection (Control group) respectively. The brain tissues of rats were collected at 1, 3, 5, 7 and 14 d after treatment. And then, immunohistochemical method and Western Blotting were used to detect expression of AQP-4, dry and wet ratio method was used to detect water content of brain. LSD method was performed for statistical analysis.　Results　①AQP - 4 was found in the pia mater, cerebral cortex, choroid plexusand the area surrounding the hemorrhage, which had been expressed in astrocytes together with GFAP . ②Water content of each group begun to increase at 3d, quickly peaked at 5d, and then gradually decreased. ③AQP - 4 of the control group and Ea group begun to increase at 3d, peaked at 5d, and then gradually decreased similar to the water content; Nevertheless, it peaked again at 14d on the BMSCs group and Ea- BMSCs group. ④Expression of AQP-4 in the Ea-BMSCs group was lower than the control group at 5 d and 7 d（P＜0.05）; There was no difference when compared with the BMSCs group. At 14d, Expression of AQP-4 in the Ea-BMSCs group was higher than the control group and BMSCs group（P＜0.05）.　Conclusion　Electro-acupuncture combined with BMSCs transplantation can significantly reduce the brain water content,which is probably correlated with up-regulation AQP-4.

Key words: Intracerebral hemorrhage, Electro-acupuncture, Bone marrow mesenchymal stem cells, Aquaporins-4

FAN Guang-Bi， XIAN De-Hai， DENG Li，TANG Hua-Jun，ZHANG Yan-ru, YANG Chao-Xian. Effects of electro-acupuncture combined with BMSCs transplantation on the expressions of AQP-4 in rats subjected to intracerebral hemorrhage[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(6): 655-660.

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Effects of electro-acupuncture combined with BMSCs transplantation on the expressions of AQP-4 in rats subjected to intracerebral hemorrhage

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