兔颈脊髓半侧挫伤模型的建立及其MRI和组织学表现的研究

doi:10.13418/j.issn.1001-165x.2017.01.007

Abstract

Abstract:

Objective 　To establish a unilateral cervical spinal cord contusion injury model in rabbits, and characterize the MRI and histology of two-level spinal cord contusion at 24 hours after injury. Methods　Twenty-two male New Zealand rabbits were randomly assigned into the moderate lesion group (n=9), the severe lesion group (n=9) and the sham group (n=4), respectively. All animals in the moderate group were subjected to 2.0 mm contusion at 500 mm/s on the left of C5 spinal cord, while all animals in the severe group to 2.8 mm contusion. The flat head impactor was 3.0 mm in diameter and was driven by an electromagnetic-servo material testing machine. The sham group exposed the spinal cord without contusion. Post-injury MRI was scanned and analyzed for 2 animals per group. Spinal cord samples of all animals were harvested 24 hours after injury, and analyzed the hemorrhagic area of the cord on cross section of the epicenter.　 Result　The peak force and actual contusion displacement were (2.47±0.39) N and (1.99±0.02) mm in the moderate group, and (5.16±0.82) N and (1.99±0.02) mm in the severe group, respectively. There was significant difference in the peak force between groups (P<0.05). There were high and low intensity T2-weighted signal in the C5 cord on post-operative MRI in the two injured groups. Histological analysis showed that there were hematoma and tissue damage on the left side of spinal cord in two injured groups, and hHematoma area was (0.012±0.006) mm2 in the moderate group and (0.039±0.006) mm2 in the severe group. There was significant difference in hematoma area between groups (P<0.05).　Conclusion　The study establishes a unilateral cervical spinal cord contusion model with displacement control and at high speed. Different unilateral cervical spinal cord contusions showed different degrees of lesions in peak force, MRI and histology.

Key words: Cervical spinal cord injury, unilateral contusion, rabbit, MRI, histology

LIU Jun-hao, YAO Xin-qiang, HUANG Zu-cheng, HUANG Zhi-ping, WU Xiao-liang, WU Xiu-hua, JI Wei, KONG Gang-gang, LI Rong, ZHU Qing-an. A unilateral cervical spinal cord contusion injury model in rabbits and its MRI and histologic characterization[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(1): 31-36.

References

[1] Rivlin AS, Tator CH. Effect of duration of acute spinal cord compression in a new acute cord injury model in the rat[J]. Surg Neurol, 1978, 10(1):38-43.
[2] Zhang Q, Huang C, Meng B, et al. Changes in autophagy proteins in a rat model of spinal cord injury[J]. Chin J Traumatol, 2014,17(4):193-197.
[3] Yeo SJ, Hwang SN, Park SW, et al. Development of a rat model of graded contusive spinal cord injury using a pneumatic impact device[J]. J Korean Med Sci, 2004,19(4): 574-580.
[4] Simard JM, Tsymbalyuk O, Keledjian K, et al. Comparative effects of glibenclamide and riluzole in a rat model of severe cervical spinal cord injury[J]. Exp Neurol, 2012, 233(1): 566-574.
[5] Lee JH, Streijger F, Tigchelaar S, et al. A contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor[J]. J Vis Exp, 2012, (65): 3313
[6] Jaumard NV, Leung J, Gokhale AJ, et al. Relevant Anatomic and Morphological Measurements of the Rat Spine: Considerations for Rodent Models of Human Spine Trauma[J]. Spine (Phila Pa 1976), 2015, 40(20):E1084-E1092.
[7] Kastner A, Gauthier P. Are rodents an appropriate pre-clinical model for treating spinal cord injury? Examples from the respiratory system[J]. Exp Neurol, 2008, 213(2):249-256.
[8] Tator CH. Review of treatment trials in human spinal cord injury: issues, difficulties, and recommendations[J]. Neurosurgery, 2006, 59(5):957-987.
[9] Sparrey CJ, Salegio EA, Camisa W, et al. Mechanical design and analysis of a unilateral cervical spinal cord contusion injury model in non-human primates[J]. J Neurotrauma, 2016, 33(12):1136-1149.
[10] Salegio EA, Bresnahan JC, Sparrey CJ, et al. A unilateral cervical spinal cord contusion injury model in non-human primates (Macaca mulatta)[J]. J Neurotrauma, 2016, 33(5):439-459.
[11] Ma Z, Zhang YP, Liu W, et al. A controlled spinal cord contusion for the rhesus macaque monkey[J]. Exp Neurol, 2016, 279:261-273.
[12]Guizar-Sahagun G, Grijalva I, Hernandez-Godinez B, et al. New approach for graded compression spinal cord injuries in Rhesus macaque: method feasibility and preliminary observations[J]. J Med Primatol, 2011, 40(6):401-413.
[13] Anderson KD. Targeting recovery: priorities of the spinal cord-injured population[J]. J Neurotrauma, 2004, 21(10):1371-1383.
[14] Ozawa H, Wu ZJ, Tanaka Y, et al. Morphologic change and astrocyte response to unilateral spinal cord compression in rabbits[J]. J Neurotrauma, 2004, 21(7):944-955.
[15] Marquardt G, Setzer M, Theisen A, et al. Experimental subacute spinal cord compression: correlation of serial S100B and NSE serum measurements, histopathological changes, and outcome[J]. Neurol Res, 2011, 33(4): 421-426.
[16] 姜杰，丁寅，周剑，等. 大鼠颈椎骨折错位致脊髓损伤模型的继发性损伤研究[J]. 中国临床解剖学杂志, 2013,31(2):165-169.
[17] 周剑，王晓萌，刘祺，等. 脊髓挫伤速度对颈脊髓原发性损伤影响的实验研究[J]. 中国临床解剖学杂志, 2014, 32(2):179-183.
[18] Ma L, Zhang D, Chen W, et al. Correlation between magnetic resonance T2 image signal intensity ratio and cell apoptosis in a rabbit spinal cord cervical myelopathy model[J]. Chin Med J (Engl), 2014, 127(2):305-313.

A unilateral cervical spinal cord contusion injury model in rabbits and its MRI and histologic characterization

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Li Wen, Yang Siyi, Huang Lei, Qing Jiwen, Jiang Songtao, Zhang Lei. Morphological characteristics and clinical significance of Lisfranc ligament based on MRI [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 21-25.
[2]	Lv Juan, Xu Shili, Lu Jing. Diagnostic value of CTA and HR-MRI in young stroke patients with arterial dissection [J]. Chinese Journal of Clinical Anatomy, 2023, 41(3): 360-364.
[3]	Lu Yijia , Chen Chunlin , Liu Yunlu, Feng Jie , Liu Ping. Explore the long-term axial changes of vagina after radical hysterectomy by three-dimensional visualization [J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 137-142.
[4]	Sun Shanshan, Tian Mimi, Zhao Peng, Cai Shuo, Yang Tao, Yang Yongqing, Ma Wenjing, Lin Xiangtao. MRI study of the effect of isolated unilateral ventriculomegaly on the volume of brain parenchyma [J]. Chinese Journal of Clinical Anatomy, 2023, 41(1): 15-19.
[5]	Ma Xueqiang, Cao Linzhong, Jiang Wei, Zhang Qi , Liu Mengchu, Wang Duoxian. Effects of Shenggu Zaizao Pills on related indexes of bone morphometry in steroid-induced necrosis of the femoral head in rabbits [J]. Chinese Journal of Clinical Anatomy, 2022, 40(5): 555-561.
[6]	Deng Bo, Xu Shuqin, Wang Fangzhou, Shi Yuhang, Li Yixing, Lin Yi, Fan Qian, Ji Lin, Chen Wenwen, Lv Yi, Yan Xiaopeng. Experimental study on the treatment of distal ureteral obstruction by magnetic compression technique [J]. Chinese Journal of Clinical Anatomy, 2021, 39(3): 319-322.
[7]	Chen Lan, Chen Chunlin, Liu Ping, Feng Jie, Wang Jianping, Tang Lian, Liao Kedan, Jiang Wenxuan, Liang Shiqi. Digital fine anatomy of bladder and urethra in rest state of young nullipara with a normal pelvic floor function [J]. Chinese Journal of Clinical Anatomy, 2021, 39(1): 31-36.
[8]	Li Zhen, Huang Hai, Liao Jianwen, Fan Shicai, Yang Cheng. Imaging and histology research of citrate-based biodegradable screws [J]. Chinese Journal of Clinical Anatomy, 2021, 39(1): 60-64.
[9]	XIE Jin-hua, ZHANG Jian-he, WANG Jia-xing, WEI Liang-feng, ZHANG Hui-jian, YUAN Bang-qing, WANG Shou-sen. Imaging features of the anterior intercavernous sinus in MRI [J]. Chinese Journal of Clinical Anatomy, 2020, 38(5): 511-517.
[10]	YOU Yun-feng, WANG Ming-liang, ZENG Meng-su. MRI manifestations of intrahepatic portal-systemic venous shunt [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 396-400.
[11]	LIN Qiong-ping, LI Da-fei, YANG Jing-ya, XU Piao, ZHANG Shan-shan, TIAN Guang-yong. Anatomical study on middle ear of New Zealand rabbits by transcanal endoscopy [J]. Chinese Journal of Clinical Anatomy, 2020, 38(1): 14-18.
[12]	BAI Gui-qin, XIAO Ru-hui, CHEN Li, HUANG Xiao-hua, YANG Zheng-wei, CAI Chang-ping. Sectional imaging study of the hippocampus in patients with alzheimer's disease [J]. Chinese Journal of Clinical Anatomy, 2020, 38(1): 29-34.
[13]	QIAN Lei, WANG Yi-ning, LIANG Wen-jie, FENG Zheng-kuan, MA Dong, OUYANG Jun. Accuracy of ischiofemoral space as a diagnostic index for ischiofemoral impingement syndrome: a meta-analysis [J]. Chinese Journal of Clinical Anatomy, 2020, 38(1): 97-101.
[14]	LI Pei, HOU Xin-xin, XU Xin-chao, FU Sheng-qi. Diffusion tensor imaging observation and clinical application of the lumbar intervertebral disc [J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 512-516.
[15]	LI Pei, LI Pin-pin, ZU Xiang-yang, FU Sheng-qi. Diffusion tensor imaging observation and clinical application of the cisternal segment of trigeminal nerve [J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 381-385.