施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响

doi:10.13418/j.issn.1001-165x.2017.01.010

Abstract

Abstract:

Objective　To investigate the effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury (SNI) and explore the protective effects of Schwann-like cells on spinal ganglion.　Methods　Firstly，the adipose derived stem cells (ADSCs) were differentiated into Schwann-like cells and the phenotype of the differentiated cells were identified, then the ADSCs and Schwann-like cells were implanted into the acellular nerve grafts (ANA), so the tissue engineering nerves were constructed. The rats were randomly divided into 3 groups: normal control group, ADSC group and Schwann-like cells group. The SNI model was established in rats of the latter two groups, and then the corresponding tissue engineering nerves were used to bridge the injured sciatic nerves. 4 Weeks after operation, Western Blot and Real-time PCR were used to detect the expression of NGF and BDNF protein and mRNA in spinal ganglia.　Results　ADSCs can be induced to differentiate into Schwann-like cells and expression of S100β and GFAP protein (markers of Schwann cells). In contrast with ADSC group, the expressions of NGF and BDNF protein and mRNA in Schwann-like cells group rats increased (P＜0.05). Conclusion　 Schwann-like cells can enhance the expression of NGF and BDNF in the spinal ganglia, so they can have protective effects on the spinal ganglia in rats with SNI.

Key words: Schwann-like cells, Spinal ganglia, Nerve growth factor, Brain-derived neurotrophic factor

References

[1] Liu G, Cheng Y, Guo S, et al. Transplantation of adipose-derived stem cells for peripheral nerve repair[J]. Int J Mol Med, 2011, 28(4):565-572.
[2] Zheng M, Duan J, He Z, et al. Overexpression of tropomyosin receptor kinase A improves the survival and Schwann-like cell differentiation of bone marrow stromal cells in nerve grafts for bridging rat sciatic nerve defects[J]. Cytotherapy, 2016,18(10):1256-1269.
[3] Fu X, Tong Z, Li Q, et al. Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation[J]. Mol Med Rep, 2016,14(2):1187-1193.
[4] 崔慧先. 系统解剖学[M]. 北京：人民卫生出版社，2009：304-319.
[5] Wu Y, Wang L, Guo B, et al. Electroactive biodegradable polyurethane significantly enhanced Schwann cells myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering[J]. Biomaterials, 2016,87:18-31.
[6] Li HF, Wang YR, Huo HP, et al. Neuroprotective effects of ultrasound-guided nerve growth factor injections after sciatic nerve injury[J]. Neural Regen Res, 2015, 10(11):1846-1855.
[7] Li S, Wang X, Gu Y, et al. Let-7 microRNAs regenerate peripheral nerve regeneration by targeting nerve growth factor[J]. Mol Ther, 2015, 23(3):423-433.
[8] Xu C, Bai L, Chen Y, et al. Effect of mutated defensin NP-1 on sciatic nerve regeneration after transection-A pivot study[J]. Neurosci Lett, 2016,617:283-287.
[9] Hannan JL, Albersen M, Stopak BL, et al. Temporal changes in neurotrophic factors and neurite outgrowth in the major pelvic ganglion following cavernous nerve injury[J]. J Neurosci Res, 2015, 93(6):954-963.
[10]Lin G, Zhang H, Sun F, et al. Brain-derived neurotrophic factor promotes nerve regeneration by activating the JAK/STAT pathway in Schwann cells[J]. Transl Androl Urol, 2016, 5(2):167-175.
[11]Zheng J, Sun J, Lu X , et al. BDNF promotes the axonal regrowth after sciatic nerve crush through intrinsic neuronal capability upregulation and distal portion protection[J]. Neurosci Lett, 2016, 621:1-8.

Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury

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