6-羟多巴损伤黑质导致室旁核离子钙接头蛋白表达变化

doi:10.13418/j.issn.1001-165x.2017.02.014

Chinese Journal Of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2): 188-192.doi: 10.13418/j.issn.1001-165x.2017.02.014

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Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine

ZHANG Yi-min ^1,3, LIAN Hui ^2,3, FU Sheng-qi¹, REN Tong-ming², CAI Jun-yan⁴, WANG Zhi-yong^1,3

1.Henan Key Laboratory for Medical Tissue Regeneration，2.Lab of Anatomy, 3.Department of Anatomy, Basic medical, Xinxiang Medical University, Xinxiang 453003, China; 4.Zhongda Hospital, Southeast University, Nanjing 210009, China

Received:2016-11-23 Online:2017-03-25 Published:2017-04-27

Abstract

Abstract:

Objective　To observe the expression of ionized calcium binding adapter molecule-1 (Iba1) in rat paraventricular nucleus of hypothalamus (PVN) induced by substantia nigra lesion by 6-hydroxydopamine (6-OHDA).　Methods　According to random number table, a total of 30 male SD rats were randomly divided into a 6-OHDA group and a normal control group. 6-OHDA was injected into the bilateral substantia nigra (SN) of the rats in the 6-OHDA group, and the same volume of saline was injected into the same position of the rats in the normal control group. After sixweeks, the rats were sacrificed and the brains were obtained. Nissl staining was used to detect the Nissl bodies in the SN, and immunofluorescent assay and Western blot analysis were used to detect TH expression in the SN and Iba1 expression in the PVN, and RT-PCR were used to detect Iba1 in the PVN.　Results　Compared with the normal control group, the number of TH immunoreactive neurons in the SN of the 6-OHDA group decreased from (58±5) to (10±2) (P<0.05), and the protein level of TH decreased from (0.71±0.1) to (0.28±0.12) (P<0.05). The number of Iba1 immunoreactive cells in the PVN of the 6-OHDA group increased from (7±2) to (14±3) (P<0.05), and the level of Iba1 protein increased from (0.1±0.08) to (0.19±0.06) (P<0.05).　Conclusion　The changes of Iba1 expression in PVN may be associated with gastric emptying dysfunction in patients with Parkinson’s disease.

Key words: Parkinson's disease, Paraventricular nucleus of hypothalamus, Ionized calcium binding adapter molecule-1

ZHANG Yi-min, LIAN Hui, FU Sheng-qi, REN Tong-ming, CAI Jun-yan, WANG Zhi-yong. Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(2): 188-192.

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Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine

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