低强度高频率振动对成骨细胞生物学特性的影响

doi:10.13418/j.issn.1001-165x.2018.02.010

Abstract

Abstract:

Objective　To study the effects of low-magnitude high-frequency vibration (LMHFV) on the biological characteristics of osteoblasts.　Methods　To establish a model of MC3T3-E1 cells loaded with LMHFV, and observe the effects of LMHFV at different frequencies on the concentration ratio of OPG / RANKL in MC3T3-E1 cells, the frequency with the highest OPG / RANKL concentration ratio (F) was the frequency of follow-up studies. With 0 Hz as a control, to observe the effects of LMHFV on the expression of akialine phosphatase (ALP), osteocalcin (OCN) mRNA and protein activity, and calcified nodules in MC3T3-E1 cells; RAW264.7 cells were incubated with conditioned medium (CMF) loaded with LMHFV, and to observe the effect of CMF on TRAP staining, multinucleated osteoclast formation, TRAP mRNA and protein activity in osteoclasts; to study the expression of cyclooxygenase-2 (COX-2) protein level in MC3T3-E1 cells loaded with LMHFV and the effect of COX-2 inhibitor NS-398 on the differentiation of MC3T3-E1 cells induced by LMHFV.　Results　The highest concentration ratio of OPG / RANKL was obtained at 30 Hz in LMHFV, which promoted the increase of ALP, OCN mRNA and protein activity and increased the formation of calcified nodules. CM formed by LMHFV at 30 Hz inhibited the differentiation of RAW264.7 cells into multinucleated osteoclasts and inhibited the TRAP mRNA and protein activity. LMHFV induced an increase in COX-2 protein level and NS-398 inhibited LMHFV-induced osteoblast differentiation.　Conclusion　LMHFV at 30 Hz had a positive effect on OPG / RANKL concentration ratio and osteogenic differentiation in MC3T3-E1 cells, indirectly suppresses bone resorption by regulating the osteoblast OPG / RANKL concentration ratio, the COX-2 pathway is involved in the regulation of osteoblast biological properties by LMHFV.

Key words: Osteoporosis; , Low-intensity high-frequency vibration, Osteoblast; , Biological characteristics

ZHA Ding-sheng, DENG Xuan-geng, CHEN Jian-ting. Effects of low magtitude and high frequency vibration on the biological characteristics of osteoblasts[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 165-173.

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Effects of low magtitude and high frequency vibration on the biological characteristics of osteoblasts

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