SETD4对脂多糖诱导的AML12细胞IL-6转录的调控作用

doi:10.13418/j.issn.1001-165x.2018.03.013

Abstract

Abstract:

Objective　To explore the effect of SETD4 (SET-domain containing protein 4) in IL-6 transcription in AML12 cells (murine hepatocyte line) stimulated with LPS.　Methods　Recombination plasmids encoding SETD4 or IL-6 promoter luciferase were constructed and cotransfected into AML12 cells. After LPS stimulation, the luciferase activity of IL-6 promoter was detected by Dual-luciferase reporter assay system. The LPS-induced IL-6 mRNA in AML12 cells with SEDT4 over-expression was also measured. Results　The plasmids of pcDNA3.0/HA-SETD4 and pGL3.0/IL-6 promoter were constructed successfully, evidenced by double restriction enzyme digestion identification and DNA sequencing. The results showed that SETD4 have no effect on luciferase activity when IL-6 promoter luciferase plasmid and SETD4 plasmid were cotransfected into the AML12 cells following LPS stimulation. Over-expression of SETD4 could up-regulate the LPS-induced IL-6 mRNA transcription in AML12 cells.　Conclusion　SETD4 expression plasmid and IL-6 promoter luciferase plasmid were constructed successfully. SETD4 may play a positive effect on LPS-induced IL-6 transcription in AML12 cells.

Key words: , SETD4；　LPS；　AML12；　IL-6 transcription；　Luciferase reporter gene

SUN Jiang, LI Yue, NIU Shi-xian, HUANG Meng-yi, ZHONG Yu-yun, ZHAO Shu-qi, LUO Hai-hua, JIANG Yong, LIU Jing-hua. Effect of SETD4 on LPS-induced IL-6 transcription in AML12 cells[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(3): 282-287.

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Effect of SETD4 on LPS-induced IL-6 transcription in AML12 cells

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