HuR诱导骨髓间充质干细胞成软骨分化的作用探析

doi:10.13418/j.issn.1001-165x.2018.03.014

Abstract

Abstract:

Objective　To observe the effect of HuR on chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and to explore its mechanism.　Methods　BMSCs were extracted from 4-week-old SD rats. The third generation BMSCs were divided into a HuR group, and a NC group, and were respectively treated with pAdEasy-HuR-GFP virus solution and pAdEasy-GFP virus solution. After intervention, the cells were fixed 14 days after induction, paraffin-embedded and sectioned. Toluidine blue staining, QPCR and IHC detection of XIAP, HuR, Col2a1 expression differences were performed.　Results Toluidine blue staining showed that positive signals of proteoglycan staining by HuR staining were more than NC and cartilage ball diameter was larger than NC. QPCR revealed that cartilage HuR, XIAP was significantly increased, Col2a1 up-regulation was not obvious. Immunohistochemical results showed that XIAP, HuR, Col2a1 gradually increased with the increase of drug-containing serum concentration, XIAP upward trend more obvious, HuR and Col2a1 upregulation is not obvious. QPCR detected cartilage HuR, XIAP was significantly increased, Col2a1 up-regulation is not obvious.　Conclusion　Overexpression of HuR facilitates the differentiation of BMSCs into cartilage, which may be related to the regulation of XIAP transcription after HuR over-expression.

Key words: RNA binding protein;　 HuR; , BMSC, 　Differentiation into cartilage

LI Yong-jin, WU Hui-mei, LIN Yong-peng, HOU Yu,CHEN Bo-lai. HuR induces bone marrow mesenchymal stem cells into chondrogenic differentiation[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(3): 304-308.

References

[1] Zhuang Z, Ye G, Huang B. Kaempferol alleviates the interleukin-1β-induced inflammation in rat osteoarthritis chondrocytesvia suppression of NF-κB[J]. Med Sci Monit, 2017, 14(23): 3925-3931.
[2] Jia S, Zhang T, Xiong Z, et al. In vivo evaluation of a novel oriented scaffold-BMSC construct for enhancing full-thickness articular cartilage repair in a rabbit model[J]. PLoS One, 2015, 10(12) :e0145667
[3] Mostafavinia A, Dehdehi L, Ghoreishi SK, et al. Effect of in vivo low-level laser therapy on bone marrow-derived mesenchymal stem cells in ovariectomy-induced osteoporosis of rats[J]. J Photochem Photobiol B, 2017,18(175):29-36.　　　　　　　
[4] Cao XK, Li R, Sun W, et al. Co-combination of islets with bone marrow mesenchymal stem cells promotes angiogenesis[J]. Biomed Pharmacother, 2016, 78:156-164.　　　[5] Chang N, Ge J, Xiu L, Zhao Z, et al. HuR mediates motility of human bone marrow-derived mesenchymal stem cells triggered by sphingosine 1-phosphate in liver fibrosis[J]. J Mol Med (Berl), 2017, 95(1): 69-82.
[6] Alcaraz MJ, Megías J, García-Arnandis I, et al. New molecular targets for the treatment of osteoarthritis. Biochem Pharmacol, 2010, 80(1):13-21.
[7] Han SH, Kim YH, Park MS, et al. Histological and biomechanical properties of regenerated articular cartilage using chondrogenicbone marrow stromal cells with a PLGA scaffold in vivo[J]. J Biomed Mater Res A, 2008,87(4):850-861.
[8] Chen FH, Tuan RS. Mesenchymal stem cells in arthritic diseases[J]. Arthritis Res Ther, 2008,10(5):223.
[9] Grammatikakis I, Abdelmohsen K, Gorospe M. Posttranslational control of HuR function[J]. Wiley Interdiscip Rev RNA, 2017, 8(1): 1372-1385.
[10]Ge J, Chang N, Zhao Z, et al. Essential roles of RNA-binding protein HuR in activation of hepatic stellate cells induced by transforming growth factor-β1[J]. Sci Rep, 2016, 25(6): 22141-22155.
[11] Zhang X, Zou T, Rao JN, et al. Stabilization of XIAP mRNA through the RNA binding protein HuR regulated by cellular polyamines[J]. Nucleic Acids Res, 2009 , 37(22): 7623-7637.
[12]Abdel-Magid AF. Modulation of the inhibitors of apoptosis proteins (IAPs) activities for Cancer Treatment[J]. ACS Med Chem Lett, 2017, 8(5): 471-473.
[13]Hou Y, Allan L A, Clarke PR. Phosphorylation of XIAP by CDK1-cyclin-B1 controls mitotic cell death[J]. J Cell Sci, 2017, 130(2): 502-511.
[14] Prabhu K S, Siveen K S, Kuttikrishnan S, et al. Targeting of X-linked inhibitor of apoptosis protein and PI3-kinase/AKT signaling by embelin suppresses growth of leukemic cells[J]. PLoS One, 2017, 12(7): e180895.
[15] Hikami S, Shiozaki A, Kitagawa-Juge M, et al. The role of cIAP1 and XIAP in apoptosis induced by tumor necrosis factor alpha in esophageal squamous cell carcinoma cells[J]. Dig Dis Sci, 2017, 62(3): 652-659.
[16] Bohm B, Hess S, Krause K, et al. ADAM15 exerts an antiapoptotic effect on osteoarthritic chondrocytes via up-regulation of the X-linked inhibitor of apoptosis[J]. Arthritis Rheum, 2010, 62(5): 1372-1382.
[17] Simons M, Beinroth S, Gleichmann M, et al. Adenovirus-mediated gene transfer of inhibitors of apoptosis protein delays apoptosis in cerebellar granule neurons[J]. J Neurochem, 1999, 72(1): 292-301.

HuR induces bone marrow mesenchymal stem cells into chondrogenic differentiation

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