单羧酸转运蛋白2在急性心力衰竭模型大鼠大脑皮质中的表达变化

doi:10.13418/j.issn.1001-165x.2018.03.015

Chinese Journal Of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (3): 308-312.doi: 10.13418/j.issn.1001-165x.2018.03.015

Previous Articles Next Articles

Changes of MCT2 expression in cerebral cortex of rats with acute heart failure

SONG Kai-qin¹, KANG Cheng-qiao², SUN Shan-quan³

1.Center for Physical Examination, Third Affiliated Hospital, Chongqing Medical University, Chongqing 401120, China; 2. Department of Anesthesiology, Third Affiliated Hospital, Chongqing Medical University, Chongqing 401120, China; 3.Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China

Received:2017-11-23 Online:2018-05-25 Published:2018-07-04

Abstract

Abstract:

Objective　To observe the changes of monocarboxylate transporters 2( MCT2) expression in the rat cerebral cortex under the stimulation of acute heart failure syndrome, so as to detect the alterations of energy metabolism of the cerebral cortex under the condition of acute heart failure, and to provide experimental basis for the best rescue time of acute heart failure.　Methods　The model was established by intravenous injection of 1.5% pentobarbital sodium.To estimate whether acute heart failure model was successful or not, the left ventricular systolic pressure（LVSP）and increased rate of left ventricular pressure maximum（+dp/dt max）were recorded. Immunohistochemical staining(IHC) and Western blot(WB) were used to explore the alteration of MCT2 expression in rat cerebral cortex. 　Results　Compared with the normal group, the number and the integral optical density（IOD）of MCT2-positive neurons were increased in the cerebral cortex of the acute heart failure model，reaching the highest level on 5 min and declined on 10 min, but still higher than those in the control group (P<0.05).　Conclusion　Our results involved that MCT2 is enhanced under the case of acute heart failure, which might indicate that MCT2 is involved in the process of self-compensation in the absence of cerebral ischemia.

Key words: Acute heart failure model, 　MCT2, 　Cerebral cortex

SONG Kai-qin1, KANG Cheng-qiao2, SUN Shan-quan3. Changes of MCT2 expression in cerebral cortex of rats with acute heart failure[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(3): 308-312.

References

[1] 中华医学会心血管病学分会.中国心力衰竭诊断和治疗指南2014[J].中华心血管病杂志, 2014, 42(2): 98-122.
[2] Oz G, Seaquist ER, Kumar A, et al. Human brain glycogen content and metabolism: implications on its role in brain energy metabolism[J]. Am J Physiol Endocrinol Metab, 2007, 292(3): E946-E951.
[3] 宋开琴, 康承巧, 孙善全, 等. MCT2在福尔马林致痛模型大鼠大脑皮质中的表达变化[J]. 中国临床解剖学杂志, 2014, 32(3): 312-315.
[4] BOUZIER-SORE AK, VOISIN P, CANIONI P, et al. Lactate is a preferential oxidative energy substrate over glucose for neurons in culture[J]. J Cereb Blood Flow Metab, 2003, 23(11): 1298-1306.
[5] 郭娟, 郝海智, 董海龙. 中枢内星形胶质细胞源性乳酸对神经元的“容积递质”样影响作用的研究进展[J]. 神经解剖学杂志, 2017, 33(6): 767-770.
[6] Halestrap AP, Price NT. The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation[J]. Biochem J, 1999, 343 Pt 2(2): 281-299.
[7] 曾军英,韩雪峰,谭支良.单羧酸转运蛋白家族及其生物学功能[J].生命的化学, 2008, 28(4): 404-407.
[8] 何书娟,孙善全,马容,等.单羧酸转运蛋白1、2、4在急性心衰大鼠模型肾上腺的表达变化[J].中国临床解剖学杂志, 2015, 33(2): 193-198.
[9] Halestrap AP, Meredith D. The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond[J]. Pflugers Arch, 2004, 447(5): 619-628.
[10]李文娟, 孙善全, 朱淑娟, 等. 单羧酸转运蛋白1、2在人星形胶质细胞瘤中的表达变化及其与病理级别之间的关系[J]. 解剖学杂志, 2014, 37(1): 54-57.
[11]Friesema EC, Ganguly S, Abdalla A, et al. Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter[J]. J Biol Chem, 2003, 278(41): 40128-40135.
[12]Kitano T, Nisimaru N, Shibata E, et al. Monocarboxylates and glucose utilization as energy substrates in rat brain slices under selective glial poisoning-a 31 P NMR study[M]. Springer US, 2003.
[13]Kirat D, Kato S. Monocarboxylate transporter 1 (MCT1) mediates transport of short-chain fatty acids in bovine caecum[J]. Exp Physiol, 2006, 91(5): 835-844.
[14]Pierre K, Pellerin L. Monocarboxylate transporters in the central nervous system: distribution, regulation and function[J]. J Neurochem, 2005, 94(1): 1-14.
[15]Pellerin L. Lactate as a pivotal element in neuron-glia metabolic cooperation[J]. Neurochem Int, 2003, 43(4-5): 331-338.
[16]Erlichman JS, Hewitt A, Damon TL, et al. Inhibition of monocarboxylate transporter 2 in the retrotrapezoid nucleus in rats: a test of the astrocyte-neuron lactate-shuttle hypothesis[J]. J Neurosci, 2008, 28(19): 4888-4896.

Changes of MCT2 expression in cerebral cortex of rats with acute heart failure

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Metrics

Comments

Recommended 0