Abstract:

Objective　To build the periodontal capillaries casting of Wistar rats by perfusion of their capillaries with microgranular materials, which provided a morphological method for study the microcirculation of periodontitis.　Methods　8-week-old Wistar rats were used to establish the animal model of periodontitis. Micropump was implanted from the inlet of the left ventricle of the aorta, and the liquid of the microgranular material was perfused to rely on the pressure of the pump. The swelling and redness of the skin indicated the success of perfusion. After the filling material was fully polymerized in the specimen for 24 hours, the soft tissue was removed by corrosion. The capillary cast specimens of periodontitis were  observed with the S-3000N scanning electron microscope (SEM).    Results  Under scanning electron microscope from 30, 2000, until 4500 times, the casting of the periodontal capillary, the three-dimensional space morphology and distribution of the capillaries of structures such as the gingival margin, gingival crevicular, gingival papillae  could be clearly viewed in the casting of the periodontal capillary.  Conclusion  The capillary cast can provide the morphological methods for periodontitis microcirculation research, and can be coordinated with the molecular biologic research of periodontitis, revealing various pathological changes of periodontitis from different aspects.

Key words:  , Wistar rats, 　Periodontitis, 　Capillaries casting