高尔基体基质蛋白130通过诱导上皮间充质转化促进甲状腺乳头状癌细胞TPC-1的侵袭和迁移

doi:10.13418/j.issn.1001-165x.2019.01.014

Abstract

Abstract:

Objective　To investigate the effect and mechanism of Golgi Matrix Protein 130(GM130) on the abilities of invasion and migration in papillary thyroid carcinoma cells.　Methods　The mRNA levels of GM130 in thyroid cell Nthy-ori 3-1 and papillary thyroid carcinoma cell BCPAP, K1 and TPC-1 were measured by RT-PCR. Cells were divided into Control, scramble siRNA(si-scramble), and GM130 siRNA(si-GM130) groups. CCK8 was performed to evaluate cell proliferation. Wound healing assay was performed to evaluate migration. Transwell was performed to evaluate invasion. The expression of GM130, Ki67, Proliferating cell nuclear antigen(PCNA), Matrix metalloproteinase-4(MMP-4), MMP-9, calcium-dependent adhesion protein E (E-cadherin), Snail, N-cadherin and Vimentin were measured by Western blot. Meanwhile, immunofluorescence assay was performed to evaluate the expression of Vimentin.　Results　GM130 in TPC-1 cells was higher expressed than in other papillary thyroid carcinoma cell lines. Therefore, TPC-1 cells were used for further study. Compared with the control group, the expression of GM130 was decreased in si-GM130 group, which resulted in the cell proliferation rate. And the expression of Ki67, and PCNA were decreased markedly. The wound closure rate was lower notably. The invasive cells and the expression of MMP-4, and MMP-9 declined significantly. Additionally, the expression of E-cadherin in si-GM130 group increased compared with that in control group, whereas the expression of Snail, N-cadherin and Vimentin decreased significantly. Meanwhile, the fluorescent value of Vimentin in si-GM130 group decreased significantly.　Conclusions　GM130 can promote papillary thyroid carcinoma cell TPC-1 invasion and migration through inducing epithelial-to-mesenchymal transition.

Key words: GM130, 　Epithelial-to-mesenchymal Transition, 　Papillary thyroid carcinoma, Invasion, 　Migration

CHEN Jie, TANG Li-hua, LI Zheng-ming. Golgi Matrix Protein 130 promotes papillary thyroid carcinoma cell TPC-1 invasion and migration through regulating epithelial-to-mesenchymal transition[J]. Chinese Journal Of Clinical Anatomy, 2019, 37(1): 64-70.

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Golgi Matrix Protein 130 promotes papillary thyroid carcinoma cell TPC-1 invasion and migration through regulating epithelial-to-mesenchymal transition

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