周期性牵张力介导BMP9通过PI3K/AKT信号通路调控人牙周膜成纤维细胞成骨分化

doi:10.13418/j.issn.1001-165x.2019.06.010

Abstract

Abstract: Objective　To investigate whether bone morphogenetic proteins 9 (BMP9) can activate PI3K/AKT signaling pathway to regulate osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs) under cyclic tension force.　Methods　The cultured in vitro hPDLFS were loaded with sine wave, deformation rate 10%, frequency 0.5 Hz periodic tension by FlexCell system. Distribution and changes of cytoskeletal protein expression were observed by immunofluorescence method. Changes of mRNA expression of RUNX2, OCN, OPN and OSX were observed by qPCR. Cytoskeletal protein OCN, OPN expressions and changes of AKT, P-AKT, OCN, OPN after adding P13K signal inhibitor LY294002 were detected by Western blot.　Results　Compared with control group, there were a significant increase of the expression of cytoskeletion-associated protein, up-regulation of the expression of osteogenic markers in 6 h and 12 h groups (P<0.05). The trend of mRNA expression was consistent with the result of protein detection.　Conclusions　Cyclic tension force-mediated BMP9 can regulate osteogenic differentiation of hPDLFs through PI3K/AKT signaling pathway.

Key words: BMP-9；　Cyclic tensile force, 　hPDLFs, 　PI3K/AKT

CLC Number:

R783.5

ZHANG Chao, HU Jing, ZHANG Jun-yi, WEI Ke-yuan, SUN Hai-bao, HUANG Ke-qiang. Cyclic tension force-mediated BMP9 regulates osteogenic differentiation of human periodontal ligament fibroblasts via PI3K/AKT signaling pathway[J]. Chinese Journal of Clinical Anatomy, 2019, 37(6): 656-661.

References 18

[1]	Nettelhoff L, Grimm S, Jacobs C, et al. Influence of mechanical compression on human periodontal ligament fibroblasts and osteoblasts[J]. Clin Oral Investig, 2016, 20(3):621-629.
[2]	Jacobs C, Walter C, Ziebart T, et al. Mechanical loading influences the effects of bisphosphonates on human periodontal ligament fibroblasts[J]. Clin Oral Investig, 2015, 19(3):699-708.
[3]	Pirjamalineisiani A, Sarafbidabad M, Jamshidi N, et al. Finite element analysis of post dental implant fixation in drilled mandible sites[J]. Comput Biol Med, 2016, 81(6):159-166.
[4]	Kang Q, Sun MH, Cheng H, et al. Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery[J]. Gene Ther, 2004, 11(17):1312-1320.
[5]	Luther G, Wagner ER, Zhu G, et al. BMP-9 induced osteogenic differentiation of mesenchymal stem cells: molecular mechanism and therapeutic potential[J]. Curr Gene Ther, 2011, 11(3): 229-240.
[6]	Cheng H, Jiang W, Phillips FM, et al. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs)[J]. J Bone Joint Surg Am, 2003, 85(8): 1544-1552.
[7]	Peng Y, Kang Q, Luo Q, et al. Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells[J]. J Biol Chem, 2004, 279(31): 32941-32949.
[8]	Chang M, Lin H, Fu H, et al. MicroRNA-195-5p regulates osteogenic differentiation of periodontal ligament cells under mechanical loading[J]. J Cell Physiol, 2017, 232(12): 3762-3774.
[9]	Luo Q, Kang Q, Si W, et al. Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells[J]. J Biol Chem, 2004, 279(53): 55958-55968.
[10]	杨泉林, 孟朋民, 石彬, 等. 骶韧带成纤维细胞受力后细胞骨架的变化[J]. 中国妇幼保健, 2014, 29(23): 3814-3816.
[11]	Ren D, Wei F, Hu L, et al. Phosphorylation of Runx2, induced by cyclic mechanical tension via ERK1/2 pathway, contributes to osteodifferentiation of human periodontal ligament fibroblasts[J]. J Cell Physiol, 2015, 230(10): 2426-2436.
[12]	姚依村, 梁伟国, 叶冬平, 等. 细胞骨架与力学信号传导[J]. 中国组织工程研究, 2014, 18(7): 1109-1111.
[13]	吴莉萍, 韦曦, 凌均棨, 等. 细胞骨架及相关蛋白在人牙周韧带细胞成骨分化过程中的表达模式[J]. 中华口腔医学研究杂志(电子版), 2008, 2(5): 8-12.
[14]	戚孟春, 胡静, 韩立赤, 等. 成骨细胞在机械力刺激下细胞骨架及细胞形态改变的体外研究[J]. 中国口腔颌面外科杂志, 2004, 2(3): 181-184.
[15]	Fuchigami S, Nakamura T, Furue K, et al. Recombinant human bone morphogenetic protein-9 potently induces osteogenic differentiation of human periodontal ligament fibroblasts[J]. Eur J Oral Sci, 2016, 124(2): 151-157.
[16]	Zhu JH, Liao YP, Li FS, et al. Wnt11 promotes BMP9-induced osteogenic differentiation through BMPs/Smads and p38 MAPK in mesenchymal stem cells[J]. J Cell Biochem, 2018, 119(11): 9462-9473.
[17]	Furue K, Sena K, Sakoda K, et al. Involvement of the phosphoinositide 3-kinase/Akt signaling pathway in bone morphogenetic protein 9-stimulated osteogenic differentiation and stromal cell-derived factor 1 production in human periodontal ligament fibroblasts[J]. Eur J Oral Sci, 2017, 125(2): 119-126.
[18]	Hamzehzadeh L, Atkin SL, Majeed M, et al. The versatile role of curcumin in cancer prevention and treatment: a focus on PI3K/AKT pathway[J]. J Cell Physiol, 2018, 233(10): 6530-6537.

Cyclic tension force-mediated BMP9 regulates osteogenic differentiation of human periodontal ligament fibroblasts via PI3K/AKT signaling pathway

Knowledge

Abstract

Cite this article

share this article

References 18

Related Articles 6

Metrics

Comments

Recommended 0

[1]	Xu Yidan, Wang yanting, Chen shaoFeng, Jing Xing, Xu Dan, Fangfang, Wang Hai, Xie Yun, Zhuang Yuehong. Investigation of Fasudil in its efficacy at improving flap innervation in mice [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 447-452.
[2]	Mi Xiaolu, Li Baiyan, Li Yaqin, Zhu Baoyue, Wang Weizhan. Effects of resveratrol on brain damage in rats with lacunar infarction [J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 683-688.
[3]	Li Qingqing, Xiong Jiahui, Wen Yuqing, Yang Hongsheng, Li Jindou, Fang Meng, Liu Yuwei. Protective effect of tanshinone IIA on CCl4-induced acute liver injury in mice [J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 671-676.
[4]	Ding Xu, Yin Zi, Deng Xiangmin, Zhang Yanjun, Song Xiaoli. Protective effects of Ginkgolide B on model rats with Parkinson’ s disease [J]. Chinese Journal of Clinical Anatomy, 2022, 40(4): 442-446.
[5]	JIA Ying, ZHAO Wei, ZHANG Xin-yuan, WU Rui, XIAO Bing-bing. The mechanism of Celastrol in inhibiting cell biological behavior of cervical cancer cells [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 421-427.
[6]	LIU Ming, WANG Jun , LI Li, CHENG Zhong-xia. Eucommia chlorogenic acid enhances the radiosensitivity of retinoblastoma cell line HXO-Rb44 via PI3K/AKT/Nrf-2 pathway [J]. Chinese Journal of Clinical Anatomy, 2019, 37(6): 644-649.