Wnt/β⁃catenin信号通路参与马蹄内翻足畸形形成的研究

doi:10.13418/j.issn.1001-165x.2021.05.014

Abstract

Abstract: Objective　To investigate the effect of sex determining region Y-box 9 (Sox9), β-catenin and Wnt/ β-catenin signaling pathway on the congenital talipes equinovarus (CTE) and its mechanism. Methods　SD rats at 10 days of gestation were randomly divided into an experimental group and a control group. All trans retinoic acid (135 mg/kg) was dissolved in mineral oil to make fetal rat CTE model in the experimental group. The control group was given the same amount of mineral oil. Immunohistochemistry, RT-PCR and Western blot were used to detect the expression levels of β-catenin, Sox9 and phosphorylated β-catenin-s552 in ankle soft tissue of control group and model group.　Results　Compared with the control group, HE staining showed more collagen deposition in CE model tissues, immunohistochemical results showed that the expression of β-catenin and Sox9 in the model group significantly increased. RT-qPCR result showed that the mRNA level of Sox9, β-catenin increased. Western blot result showed that the expression of P-β-catenin-s552 increased, the expression of Sox9 and β-catenin in the experimental group decreased. Conclusions　The high expression of Sox9 in the ankle tissue of rats with congenital clubfoot is regulated by Wnt / β-catenin signaling pathway, which is involved in the formation of congenital clubfoot.

Key words: Congenital talipes equinovarus, , 　Sox9, 　 Wnt signaling pathway, , , , β-catenin, , , Phosphorylated-β-catenin

CLC Number:

R363.2

Li Fenjie, Chen Xi, Li Yangjie, Liao Jiaxin, Li Zhifu, Liu Anzhuo, Zhang Yueqiu, Yan Nan, Wang Zhengdong . Wnt/β-catenin signaling pathway involved in the deformity of congenital talipes equinovarus[J]. Chinese Journal of Clinical Anatomy, 2021, 39(5): 575-578.

References 11

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Wnt/β-catenin signaling pathway involved in the deformity of congenital talipes equinovarus

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