CBX3在脑胶质瘤中的表达分析及其对胶质瘤细胞凋亡的抑制作用

doi:10.13418/j.issn.1001-165x.2022.5.11

Abstract

Abstract: Objective To clarify the correlation between CBX3 and the prognosis of glioma by analyzing the expression of CBX3 in the glioma public database. Flow cytometry and the detection of apoptosis indexes were used to analyze its effect on the apoptosis level of glioma cell lines. Methods By analyzing the data of glioma patients gene chip in TCGA and CGGA, the expression of CBX3 in different grades of glioma was compared and the relationship between CBX3 expression and prognosis of glioma patients was analyzed. Functional annotation of the CBX3 gene by GO and Pathway analysis. Finally, the effect of CBX3 gene on the apoptosis level of glioma cell lines was explored in cell lines. Results The expression of CBX3 in high-grade gliomas was significantly higher than that in low-grade gliomas (P<0.01). The prognosis of patients with high CBX3 expression was worse than that of patients with low CBX3 expression (P<0.05). At the same time, flow cytometry experiments showed that overexpression of CBX3 could significantly reduce the apoptosis level of glioblastoma cell lines U87MG and T98G. Conclusion The expression level of CBX3 is negatively correlated with the survival time of glioma. High expression of CBX3 can reduce the apoptosis level of glioblastoma cell lines, so CBX3 may be an effective indicator for judging the prognosis of glioblastoma.

Key words: Glioblastoma; , , CBX3; , , Bioinformatics analysis; , , Prognosis

CLC Number:

R730.264

Li Chiyang, Yang Fan, Yang Yue, Lu Yuntao, Zhou Qiang. The expression of CBX3 in brain glioma and its influence on the apoptosis of glioma cell[J]. Chinese Journal of Clinical Anatomy, 2022, 40(5): 562-568.

References 14

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The expression of CBX3 in brain glioma and its influence on the apoptosis of glioma cell

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