灯盏乙素通过STAT3信号对激活的BV-2小胶质细胞M2型极化的影响

doi:10.13418/j.issn.1001-165x.2023.1.08

Abstract

Abstract: Objective To investigate the role of scutellarin on the polarization of M2 microglia under the action of lipopolysaccharide (LPS) and the regulation mechanism of signal transducers and activators of transcription 3 (STAT3). Methods BV-2 microglia were divided into a control group (Con), a STAT3 inhibitor α-cyano-(3,4-dihydroxy)-N-benzylmycin-nicotinamide group (AG490), a LPS group (LPS), a LPS+AG490 group, a LPS+scutellarin pretreatment group (LPS+S), a LPS+S+AG490 group. The expression of STAT3, phosphorylated STAT3 (P-STAT3) and the M2 microglia marker interleukin 10 (IL-10) were detected by Western Blot and immunofluorescence staining. Results Western Blot results showed: the expression of P-STAT3 and IL-10 in the LPS group was significantly enhanced, compared with that in the control group (P<0.05); the expression of P-STAT3 was significantly reduced, the expression of IL-10 was obviously increased after pretreatment with scutellarin; both were significantly different from the LPS group (P<0.05). AG490 enhanced the effect of scutellarin. In addition, there was no statistical difference s in STAT3 in each group (P>0.05). Immunofluorescence staining results showed that scutellarin intervention reduced protein level of P-STAT3 in LPS-activated BV-2 microglia, enhanced protein level of IL-10. There was no statistical difference in protein level of STAT3 in each group. Conclusions Scutellarin promotes the polarization of BV-2 microglia to M2 type by inhibiting the activation of STAT3, which may be related to the effect of scutellarin in reducing neuro-inflammatory responses.

Key words: Scutellarin; , , BV-2 microglia; , , STAT3; , , Polarization

CLC Number:

R363.21

Jia Qiuye, Zhang Xiaolina, Chen Haolun, Yuan Yun, Wu Chunyun. Effect of Scutellarin on M2-type polarization in activated BV-2 microglia via STAT3 signaling[J]. Chinese Journal of Clinical Anatomy, 2023, 41(1): 36-44.

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Effect of Scutellarin on M2-type polarization in activated BV-2 microglia via STAT3 signaling

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