Abstract: Objective    To measure the anatomical distance between the insertion point of two different percutaneous retrograde superior ramus of the pubis screw and the surrounding important reproductive structure, and compare the risk of damaging the surrounding important reproductive structure during the insertion of the two insertion points, so as to provide basis for clinical safety of the insertion of the screw.    Methods    Fourteen anticorrosive adult specimens were selected. With the C arm machine monitoring, bilateral percutaneous retrograde screw needle placement was completed by hand. The entry point of A was selected at the front end of the cortex of the internal margin of the pubis, and the entry point of B was selected at the medial point of the pubic tuberculum in the ventral cortex of the pubis. Imaging monitoring equipment confirmed that all needles were located in the osseous channel. Anatomical specimens were used to expose important reproductive structures. The closest distance between the two insertion points and bilateral spermatic cord, dorsal nerve of penis, and corpus cavernosum of the penis was measured in male specimens. The closest distance between the two insertion points and round uterine ligament, the dorsal nerve of the clitoris, the base of the clitoral body, and the glans of the clitoris was measured in female specimens.   Results The average distance between the insertion point A and ipsilateral spermatic cord was (51.17±5.82)mm > the dorsal nerve of penis (20.94±4.16)mm > the contralateral spermatic cord (19.77±5.23)mm > the corpus cavernosum (19.65±7.30)mm. The average distance between the entry point A and the ipsilateral round uterine ligament was (63.12±10.26) mm > the contralateral round uterine ligament (56.11±8.20) mm > the glans of clitoris (40.72±3.30) mm > the dorsal nerve of clitoris (20.46±5.81) mm > the base of clitoral body (17.72±1.76) mm. The mean distance between the insertion point B and the contralateral spermatic cord was (32.22±8.50) mm > the dorsal nerve of penis (31.18±6.23) mm > the ipsilateral spermatic cord (27.09±5.58)mm > penile cavernous body (20.01±7.54)mm. The mean distance between the insertion point B and  the clitoral head was (52.80±5.91) mm > the dorsal nerve of clitoris (50.79±5.05) mm > the contralateral round ligament (43.40±6.70) mm > the ipsilateral round ligament (35.04±11.25) mm > the base of the clitoral body (33.99±3.86) mm.    Conclusions    The risk of injury of the contralateral spermatic cord, the dorsal nerve of penis, in male and the  dorsal nerve of clitoris,  the base of the clitoral body in female at the insertion point A is higher than that at the insertion point B. For the insertion point B, the possibility of injury to the dorsal nerve of clitoris in male is greater than that of in female, the possibility of injury to the dorsal penile nerve and the base of the clitoral body is greater than that of than the cavernosum of the penis. Caution should be paid when performing the same operation at the same insertion point.