五花血藤水煎液通过抗炎作用促进脊髓损伤后小鼠运动功能恢复

doi:10.13418/j.issn.1001-165x.2023.2.09

Abstract

Abstract: Objective To investigate the anti-inflammatory effect of sargentodoxa cuneata decoction (SCD) after spinal cord injury (SCI) and its effect on the recovery of motor function. Methods Thirty-six SPF grade mice were randomly divided into a sham operation group, a SCI group and a SCD group. The recovery of hindlimb motor function was detected by BMS (Basso mouse scale), and the expression levels of interleukin (IL) -6, IL-12A, tumor necrosis factor (TNF) -α and brain-derived neurotrophic factor (BDNF) were detected by Western blot. Immunofluorescence staining of NeuN was performed to observe the changes of neurons in anterior horn of spinal cord. The distribution of spinal cord motoneurons related to tibialis anterior muscle (TA) was observed by rAAV-retro retrograde tracing. Results Compared with SCI group, the BMS score of SCD group was significantly higher at 7 days after injury (P<0.05), and IL-6, IL-12A and TNF-α were significantly higher at 14 days after injury, while the expression of BDNF was significantly higher than that in SCI group (P<0.05). Compared with SCI group, the number of spinal cord anterior horn neurons in SCD group was significantly increased (P<0.05), and the number of rAAV-retro retrograde labeled TA related motor neurons was also significantly increased (P<0.05). Conclusions The sargentodoxa cuneata decoction can significantly down-regulate the levels of inflammatory factors IL-6, IL-12A and TNF- α to play an anti-inflammatory role and up-regulate the expression of BDNF, so as to protect motor neurons and promote the recovery of motor function in mice after spinal cord injury.

Key words: Spinal cord injury; , , Sargentodoxa cuneata; , , Anti-inflammatory effect; , , Functional recovery

CLC Number:

R651.2

Yang Dan, Song Haiwang, Liang Yinhua, Liu Guoxiang, Luo Yi, Zeng Zhu. Anti-inflammatory effect of sargentodoxa cuneata decoction promotes the recovery of motor function in mice after spinal cord injury[J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 172-177.

References 15

[1]	Formento E, Minassian K, Wagner F, et al. Electrical spinal cord stimulation must preserve proprioception to enable locomotion in humans with spinal cord injury[J]. Nat Neurosci, 2018, 21(12): 1728-1741. DOI: 10.1038/s41593-018-0262-6.
[2]	Kasinathan N, Vanathi MB, Sabrahmanyam VM, et a1. A review on response of immune system in spinal cord injury and therapeutic agents useful in treatment[J]. Curr Pharm Biotechnol, 2015, 16(1): 26-34. DOI: 10.2174/1389201015666141031121338.
[3]	Guo L, Ma R, Sun H, et al. Anti-inflammatory activities and related mechanism of polysaccharides isolated from Sargentodoxa cuneata[J]. Chem Biodivers,2018, 15(11): e1800343. DOI: 10.1002/cbdv. 2018 00343.
[4]	Zhang W, Sun C, Zhou S, et al. Recent advances in chemistry and bioactivity of Sargentodoxa cuneata[J]. J Ethnopharmacol, 2021, 270: 113840. DOI: 10.1016/j.jep.2021.113840.
[5]	Zhang Z, Yang L, Wang B, et al. Protective role of liriodendrin in mice with dextran sulphate sodium-induced ulcerative colitis[J]. Int Immunopharmacol, 2017, 11(52): 203-210. DOI: 10.1016/j.intimp.2017.09.012.
[6]	杨丹, 余德立, 余资江. 复方五花血藤对鼠视网膜缺血再灌注损伤的保护作用[J]. 眼科新进展, 2014, 34(8): 713-717. DOI: 10.13389/j.cnki.rao.2014.0195.
[7]	杨丹, 余德立, 孙宝飞, 等. 五花血藤提取物对大鼠视网膜缺血再灌注损伤中凋亡相关因子表达的影响[J]. 眼科新进展, 2020, 40(9): 806-810. DOI: 10.13389/j.cnki.rao.2020.0184.
[8]	毛水春, 崔承彬, 顾谦群. 中药大血藤化学成分和药理活性的研究进展[J]. 天然产物研究与开发, 2003, 15(6): 559-562. DOI: 10.16333/j.1001-6880.2003.06.020.
[9]	Peng PH, Ko ML, Chen CF. Epigallocatechin-3-gallate reduces retinal ischemia/reperfusion injury by attenuating neuronal nitric oxide synthase expression and activity[J]. Exp Eye Res, 2008, 86(4): 637-646. DOI: 10.1016/j.exer.2008.01.008.
[10]	Lee SH, Choi BY, Kho AR, et al. Protective effects of protocatechuic acid on seizure-induced neuronal death[J]. Int J Mol Sci, 2018, 19(1): 187-198. DOI: 10.3390/ijms19010187.
[11]	ZH Zhou, Q Weng, J Zhou, et al. Anti-inflammatory and anti-nociceptive activities of the extracts of Sargentodoxa cuneata and its effects on the model rats with pelvic inflammation[J]. Journal of Animal and Plant Sciences, 2012, 22(1): 44-50. DOI: 10.1111/j.1749-6632.2012.06479.x.
[12]	Q Liu, C Deng, S Huang, et al. Influence of DaXueTeng on pathological morphology, IL-6 and TNF-α of the rats with chronic pelvic inflammatory disease[J]. West J Tradit Chin Med, 2016, 29(07): 11-14. DOI: 1004-6852(2016)07-0011-04.
[13]	Liu Y, Zhou L J, Wang J, et al. TNF-α differentially regulates synaptic plasticity in the hippocampus and spinal cord by microglia-dependent mechanisms after peripheral nerve injury[J]. J Neurosci, 2017, 37(4): 871-881. DOI: 10.1523/JNEUROSCI.2235-16.2016.
[14]	Badner A, Vidal P M, Hong J, et al. Endogenous interleukin-10 deficiency exacerbates vascular pathology in traumatic cervical spinal cord injury[J]. J Neurotrauma, 2019, 36(15): 2298-2307. DOI: 10.1089/neu.2018.6081.
[15]	Keefe KM, Sheikh IS, Smith GM. Targeting neurotrophins to specific populations of neurons: NGF, BDNF, and NT-3 and their relevance for treatment of spinal cord injury[J]. Int J Mol Sci, 2017, 18(3): 548-554. DOI: 10.3390/ijms18030548.

Anti-inflammatory effect of sargentodoxa cuneata decoction promotes the recovery of motor function in mice after spinal cord injury

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