幼羊硬脑膜mRNA表达差异及影响颅缝处细胞迁移的信号通路探究

doi:10.13418/j.issn.1001-165x.2023.2.12

Abstract

Abstract: Objective To explore the differentially expressed genes of the suture dura compared with the normal subcranial dura and the mechanism of the suture dura mater on the migration of cranial suture cells, so as to provide theoretical basis for clinical suture premature closure. Methods Six 51-day- old Ovis aries were selected. The dura mater under the cranial suture and non-cranial suture were extracted, respectively. The second-generation sequencing was used to detect the differential expression of mRNA in the dural cells of different parts of the Ovis aries in the developmental stage. The biological information were analyzed. The changes of mRNA expression in the sequencing results were verified by Real-time PCR. Results Through the analysis of the biological information of the differential expression up-regulation genes shared by the dura mater at the suture and the dura below the skull, it was found that the up-regulation of the dura at the suture, relative to the dura below the skull, was mainly focused on cell migration, vascular bundle formation, and extracellular matrix production. The most enriched cell pathway was PI3K-AKT cell signaling pathway. Validation of the PI3K signaling pathway gene with high expression in the dura showed that the expression of PI3K-AKT cell signaling pathway in the dura below the suture was higher than that of the normal skull, and it was statistically significant (P<0.05). Conclusions The PI3K-AKT cell signaling pathway expression level in the dura at the suture is significantly increased compared with that below the skull, which may be related to the proliferation and migration function of the PI3K-AKT cell signaling pathway.

Key words: Skull development; , , Cranial suture; , Dura materm; , mRNA; , Second-generation sequencing technology

CLC Number:

R651

Wang Menghui, GaoYan, Ye Xiaojian, Zhang Chunyang, Shao Guo, Wu Qirun. Analysis on the differential expression of mRNA in the dura mater of Ovis aries and the potential signaling pathways affecting cell migration[J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 187-193.

References 15

[1]	Cornelissen M, Ottelander Bd, Rizopoulos D, et al. Increase of prevalence of craniosynostosis[J]. J Craniomaxillofac Surg, 2016,44(9): 1273-1279. DOI: 10.1016/j.jcms.2016.07.007.
[2]	Twigg SR, Wilkie AO. Ac-pathophysiological framework for craniosynostosis[J]. Am J Hum Genet, 2015, 97(3): 359-377. DOI: 10.1016/j.ajhg.2015.07.006.
[3]	Calpena E, Wurmser M, McGowan SJ, et al. Unexpected role of SIX1 variants in craniosynostosis: expanding the phenotype of SIX1-related disorders [J]. J Med Genet, 2021, 59(2): 165-169. DOI: 10.1136/jmedgenet-2020-107459.
[4]	Maruyama T. Stem cells of the suture mesenchyme in craniofacial bone development, repair and regeneration[J]. Keio J Med, 2019, 68(2): 42. DOI: 10.1038/ncomms10526.
[5]	Holmes G, Gonzalez-Reiche AS, Lu N, et al. Integrated transcriptome and network analysis reveals spatiotemporal dynamics of calvarial suturogenesis[J]. Cell Rep, 2020, 32(1): 107871. DOI: 10.1016/j.celrep.2020.107871.
[6]	Maruyama T, Jeong J, Sheu TJ, et al. Stem cells of the suture mesenchyme in craniofacial bone development, repair and regeneration[J]. Nat Commun, 2016, 7: 10526. DOI: 10.1038/ncomms10526.
[7]	Bala K, Cuellar A, Herren AW, et al. Identification of differentially expressed proteins between fused and open sutures in sagittal nonsyndromic craniosynostosis during suture development by quantitative proteomic analysis[J]. Proteomics Clin Appl, 2021, 15(2-3): e2000031. DOI: 10.1002/prca.202000031.
[8]	Bhat A, Boyadjiev SA, Senders CW, et al. Differential growth factor adsorption to calvarial osteoblast-secreted extracellular matrices instructs osteoblastic behavior[J]. PLoS One, 2011, 6(10): e25990. DOI: 10.1371/journal.pone.0025990.
[9]	Sahar DE, Behr B, Fong KD, et al. Unique modulation of cadherin expression pattern during posterior frontal cranial suture development and closure[J]. Cells Tissues Organs, 2010, 191(5): 401-413. DOI: 10.1159/000272318.
[10]	Betances EM, Mendez MD, M Das J. Craniosynostosis. In: StatPearls. Treasure Island (FL)[M]. StatPearls Publishing; August 12, 2021.
[11]	Gagan JR, Tholpady SS, Ogle RC. Cellular dynamics and tissue interactions of the dura mater during head development[J]. Birth Defects Res C Embryo Today, 2007, 81(4): 297-304. DOI: 10.1002/bdrc.20104.
[12]	Ogle RC, Tholpady SS, McGlynn KA, et al. Regulation of cranial suture morphogenesis[J]. Cells Tissues Organs, 2004, 176(1-3): 54-66. DOI: 10.1159/000075027.
[13]	Zhao H, Feng J, Ho TV, et al. The suture provides a niche for mesenchymal stem cells of craniofacial bones[J]. Nat Cell Biol, 2015, 17(4): 386-396. DOI: 10.1038/ncb3139.
[14]	Yu M, Ma L, Yuan Y, et al. Cranial suture regeneration mitigates skull and neurocognitive defects in craniosynostosis[J]. Cell, 2021, 184(1): 243-256.e18. DOI: 10.1016/j.cell.2020.11.037.
[15]	He F, Soriano P. Dysregulated PDGFR α signaling alters coronal suture morphogenesis and leads to craniosynostosis through endochondral ossification[J]. Development, 2017, 144(21): 4026-4036. DOI: 10.1242/dev.151068.

Analysis on the differential expression of mRNA in the dura mater of Ovis aries and the potential signaling pathways affecting cell migration

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