miRNA-130a-3p通过调控NF-κB信号通路影响损伤心肌细胞

doi:10.13418/j.issn.1001-165x.2023.2.15

Abstract

Abstract: Objective To investigate the role and possible mechanism of miRNA-130a-3p in cardiomyocyte injury induced by LPS. Methods H9C2 cells were divided into the following four groups: a normal control group, a LPS model group, a miRNA negative control group and a miRNA-130a-3p mimics group. The expression level of miRNA-130a-3p mRNA in each group was detected by RT-qPCR. The CCK-8 assay was used to detect the cell activity in each group. The contents of TNF-α, IL-6 and IL-1β were detected by ELISA assay. Western blot was used to detect the protein expression levels of NF-κBp65, IκBα, Bax, Bcl-2 and Cleaved-Caspase-3. Results The results of RT-qPCR showed that the level of miRNA-130a-3p mRNA in the LPS model group was significantly lower than that in normal control group. The results of CCK-8 showed that the cell activity of LPS group was significantly lower than that of normal control group, while compared with the LPS group, the cell activity in miRNA-130a-3p mimics group increased significantly. The results of ELISA showed that the contents of TNF-α, IL-6 and IL-1β in cell culture medium of LPS group were significantly higher than those of normal control group, while compared with the LPS group, the contents of TNF-α, IL-6 and IL-1β in cell culture medium of miRNA-130a-3p mimics group decreased significantly. The results of Western blot showed that compared with normal control group, the protein expression levels of NF-κBp65, Bax and Cleaved-Caspase-3 increased significantly in the LPS group , while the protein expression levels of IκBα and Bcl-2 decreased significantly. Compared with the LPS group, the protein expression levels of NF-κBp65, Bax and Cleaved-Caspase-3 decreased significantly, while the protein expression levels of IκBα and Bcl-2 increased significantly in miRNA-130a-3p mimics group. Conclusions miRNA-130A-3P can inhibit cell apoptosis, improve cell activity and reduce the release of inflammatory factors, so as to alleviate cardiomyocyte injury induced LPS, through inhibiting the activation of NF-κB signaling pathway.

Key words: miRNA-130a-3p; , , NF-κB signaling pathway; , , Cell injury; , , Apoptosis

CLC Number:

R329.2

Miao Yingying, An Leilei, Fu Shengqi. Effects of miRNA-130a-3p on cardiomyocyte injury through regulating NF-κB signaling pathway[J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 207-211.

References 14

[1]	Singer M, Deutschman CS, Seymour CW, et al. The third international consensus definitions for sepsis and septic shock (Sepsis-3)[J]. JAMA, 2016, 315(8): 801-810. DOI: 10.1001/jama.2016.0287.
[2]	Kolhe R, Hunter M, Liu S, et al. Gender-specific differential expression of exosomal miRNA in synovial fluid of patients with osteoarthritis[J]. Sci Rep, 2017, 7(1): 2029. DOI: 10.1038/s41598-017-01905-y.
[3]	赵轶男, 孙强, 毕龙, 等. miR-130a-3p调控SOX4对骨关节炎软骨细胞增殖、分化和炎症因子释放的影响[J]. 现代生物医学进展, 2019, 19(22): 4201-4207. DOI: 10.13241/j.cnki.pmb.2019.22.001.
[4]	王和杰, 范忠诚, 金旭红, 等. 长链非编码RNA靶向微小RNA130a-3p基因对骨关节炎软骨细胞增殖及炎性因子分泌的影响[J]. 中华实验外科杂志, 2021, 38(5): 871-873. DOI: 10.3760/cma.j.cn421213-20201106-01923.
[5]	许德星, 王伟, 张静怡, 等. miR-130a-3p靶向TRIM37蛋白缓解H2O2诱导的心肌损伤[J]. 华中科技大学学报（医学版）, 2021, 50(1): 14-20. DOI: 10.3870/j.issn.1672-0741.2021.01.003.
[6]	周涛, 杨富瑜, 高士豪, 等. 微小RNA-130a-3p在心肌梗死大鼠心肌组织的表达及其对炎症的影响[J]. 中华实验外科杂志, 2021, 38(8): 1459-1462. DOI: 10.3760/cma.j.cn421213-20210106-01001.
[7]	Weiss SL, Asaro LA, Flori HR, et al. Multiple organ dysfunction in children mechanically ventilated for acute respiratory failure[J]. Pediatr Crit Care Med, 2017,18(4): 319⁃329. DOI: 10.1097/PCC.000000 0000001091.
[8]	Zhang N, Feng H, Liao HH, et al. Myricetin attenuated LPS induced cardiac injury in vivo and in vitro[J]. Phytother Res, 2018, 32(3): 459-470. DOI: 10.1002/ptr.5989.
[9]	刘安雷, 刘聚源, 徐军, 等. 交感神经兴奋与树突状细胞活化加剧脂多糖诱导大鼠心肌损伤[J]. 基础医学与临床, 2018, 38(7): 973-977. DOI: 10.3969/j.issn.1001-6325.2018.07.014.
[10]	雷家秀, 朱雅萍, 张雨. 依托咪酯上调miR-290-5p减轻LPS诱导的大鼠心肌细胞系H9C2 损伤[J]. 基础医学与临床, 2021, 41(6): 848-853. DOI: 10.3969/j.issn.1001-6325.2021.06.012.
[11]	邢若, 王鹏, 李兴杰. 山药多糖对LPS诱导的心肌H9C2细胞炎症因子表达和细胞凋亡的影响及机制[J]. 中国免疫学杂志, 2021, 37(6): 683-688. DOI: 10.3969/j.issn.1000-484X.2021.06.009.
[12]	雷肖蠢, 张海良, 丁鹏. LncRNA MFI2-AS1靶向miR-331-3p调节LPS诱导的心肌细胞损伤[J]. 河北医药, 2021, 43(8): 1142-1146. DOI: 10.3969/j.issn.1002-7386.2021.08.004.
[13]	Huang ZQ, Xu W, Wu JL, et al. MicroRNA-374a protects against myocardial ischemia-reperfusion injury in mice by targeting the MAPK6 pathway[J]. Life Sci, 2019, 232: 116619. DOI: 10.1016/j.lfs.2019.116619.
[14]	Shi J, Bei Y, Kong X, et al. miR-17-3p contributes to exercise-induced cardiac growth and protects against myocardial ischemia-reperfusion injury[J]. Theranostics, 2017, 7(3): 664-676. DOI: 10.7150/thno.15162.

[1]	Jiang Jianhong, Duan Renpeng, Li Xiaofeng. Application of three-dimensional visualization technology in the anatomical variations of hilar bile ducts in Chinese population [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 1-4.
[2]	Guo Hongzhi, Wang Yu, Zhang Jiaqi, Liang Haibin, Ma Ziwei, Feng Wei, Wu You, Si Ziyi. Evaluation of placental vascular structure in preeclampsia by vascular casting combined with CT three-dimensional model [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 5-10.
[3]	Li Jiawei, Zhang Jing, Li Canran, Lan Wenjie, Ji Qingyu, Guo Zhiyong, Zhang Yunfeng, Liu Qi, Chen Qingwei, Li Xiaohe. X-ray measurement of proximal femur anatomical parameters in Mongolian population [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 11-16.
[4]	. Digital measurement and clinical significance of occipitocervical Angle and posterior occipitocervical Angle in children and adolescents [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 17-20.
[5]	Li Wen, Yang Siyi, Huang Lei, Qing Jiwen, Jiang Songtao, Zhang Lei. Morphological characteristics and clinical significance of Lisfranc ligament based on MRI [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 21-25.
[6]	Li Fanfan, Xu Yang, Wang Xiaoxu. Therapeutic effect of shikonin on the treatment of granuloma lobular mastitis in model rats by regulating Nrf2/HO-1 signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 26-32.
[7]	Wang Xinghang, Ding Jiayuan, Li Fang, Bao Cuifen, Yan Lijing. Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 33-41.
[8]	Cheng Yingying, Wu Jianjun, Cai Haiyan , Jiao Xunwen, Ma Jiangbo, Ding Yinxiu. Activation and functional changes of astrocytes cultured in vitro under inflammatory stimulation [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 42-46.
[9]	Qi Zhi, Yang Li, Jia Qiuye, Chen Haolun, Duan Zhaoda, Wu Chunyun. Edaravone regulates Sirt3 expression in lipopolysaccharide-activated microglia via MAPKs signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 47-53.
[10]	Zhang Jingjing, Wang Hongxin. Protective effect of Baicalin on rats with pulmonary hypertension through PDGF/P38 MAPK signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 54-58.
[11]	Yue Qin, Chen Rongli, Xiong Jingxi, Wang Tingyi, Cai Zhiheng, Yi Qiushi, Zeng Xinyi. Effect of Bletilla striata polysaccharides on the choke-area of cross-boundary flap in rats [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 59-64.
[12]	Li Xiaoyu , Zhang Lei , Fu Lei , Li Dongbo , Wang Guoyou. Finite element study on the treatment of Sanders type II calcaneal fracture with three-step reduction method [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 65-70.
[13]	Wu Yanfei, Ma Jianxiong, Lu Bin, Wang Ying, Bai Haohao, Jin Hongzhen, Ma Xinlong. Study on the correlation between the reconstruction of grayscale values using CT post-processing technology and the compressive strength of the endplate [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 71-76.
[14]	Zhuang Wanqiang, Tang Yi, Luo Yonggang, Zhang Hui. An early and mid-term retrospective study of the effects of arthroscopy combined with high tibial osteotomy on patellar position and patellofemoral joint function [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 77-82.
[15]	Luo Ying, Dou Weiyu, Wu Xiaohang, Chen Jingkun, Peng Changgui, Pan Jianying . Risk factors of postoperative complications of calcaneal fractures treated with hippocampal plate internal fixation through minimally invasive method [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 83-88.

Effects of miRNA-130a-3p on cardiomyocyte injury through regulating NF-κB signaling pathway

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 14

Related Articles 15

Metrics

Comments

Recommended 0