四氢生物蝶呤对胰腺癌细胞增殖、迁移及凋亡的影响

doi:10.13418/j.issn.1001-165x.2023.6.13

Abstract

Abstract: Objective To investigate the effect of tetrahydrobiopterin (BH4) on the biological behavior of pancreatic cancer cells and its possible mechanism. Methods The cells were divided into a control group and an experimental group. The experimental group was divided into 4 subgroups according to the concentration of BH4 (2 μM, 4 μM, 8 μM, 12 μM BH4 groups). The ability of cell proliferation, apoptosis and migration were detected by CCK-8 assay, Hoechst assay and scratch assay. The expression of Notch1 mRNA was detected by RT-qPCR and the expressions of E-cadherin and N-cadherin were detected by Western blotting. Results Compared with the control group, with the increasing of BH4 concentration, the activity and migration ability of HPAC and Bxpc-3 cell decreased and the apoptosis rate increased, and the effect of BH4 was significant at 8 μM and 12 μM (P<0.01，P<0.05). Compared with the control group, Notch1 mRNA was significantly decreased in experimental group (BH4 12 μM) (P<0.05), the expression level of E-cadherin increased (P<0.01, P<0.05) , while that of N-cadherin decreased (P<0.05). Conclusions BH4 can inhibit the proliferation, migration and promote the apoptosis of pancreatic cancer cells. The mechanism may be related to the inhibition of epithelial-mesenchymal transition (EMT) process, while Notch1 changes during EMT. It suggests that the Notch1 signaling pathway may be involved in the regulation of this process.

Key words: , BH4, , , , Pancreatic cancer, , , Epithelial-mesenchymal transition, , , Notch signaling pathway

CLC Number:

R361.3

Ma Wenting, Zhang Chen, Xie Qing. Effects of BH4 on proliferation, migration and apoptosis of pancreatic cancer cells[J]. Chinese Journal of Clinical Anatomy, 2023, 41(6): 704-708.

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Effects of BH4 on proliferation, migration and apoptosis of pancreatic cancer cells

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