Abstract: Objective   To investigate the effect of edaravone on Sirt3 and MAPKs pathway-related proteins in LPS-activated microglia and its regulatory mechanism. Methods  Western Blot and immunofluorescence were used to detect the effect of edaravone on the protein expression of Sirt3 and MAPKs pathway-related proteins ERK1/2, P38, JNK as well as their phosphorylated forms p-ERK1/2, p-P38 and p-JNK in the activated BV2 cells. Western Blot techniques were applied to detect the effects of ERK1/2 pathway inhibitor on the expressions of p-ERK1/2 and Sirt3.   Results   The expressions of Sirt3, p-ERK1/2, p-P38 and p-JNK in microglia were significantly increased after BV2 cells activation. Edaravone promoted the expression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38. The protein expression of Sirt3 and p-ERK1/2 decreased after co-treatment with edaravone and ERK1/2 inhibitor. Edaravone promoted the overexpression of Sirt3 and p-ERK1/2 and downregulated the expression of p-JNK and p-P38. Conclusions    Edaravone can regulate MAPKs signaling pathway and promote Sirt3 production in BV2 cells, possibly regulates neuroinflammatory responses via ERK/Sirt3 signaling pathway.

Key words: Edaravone; ,  , Sirt3; ,  , Microglia; ,  , MAPKs signaling pathway