白芨多糖对大鼠跨区皮瓣choke区的影响

doi:10.13418/j.issn.1001-165x.2024.1.11

Abstract

Abstract: Objective To investigate the effect and mechanism of Bletilla striata polysaccharides on the choke-area of cross-boundary skin flap in rats. Methods Thirty-two SD rats were randomly divided into two groups: a control group, was given physiological saline; a treatment group, was given physiological saline solution of Bletilla striata polysaccharides. The thoracodorsal artery and posterior intercostal artery were ligated surgically, preserving the iliac lumbar artery. The survival rate of the flap was counted at 7 days after operation. The growth of choke artery was observed by angiography. The microvessel density and arterial intraluminal diameter of choke area were observed by HE staining microscope, and the expression level of vascular endothelial growth factor (VEGF) protein in the flap tissue of choke area was detected by Western blot. Results At day 7 after operation, the survival rate of the flap in the treatment group was higher than that in the control group[(86.1±1.4)% vs (61.3±4.0)%, P<0.0001].The diameter of the iliopsoas artery in the treatment group was thicker than that of the control group(681.60±35.48 vs 446.98±21.61μm, P<0.0001), and the blood vessels extended longer towards the distal end of the flap(6.50±0.33 vs 5.41±0.38 cm，P<0.0001). The microvessel density in choke area was higher in the treatment group [(11.8±1.5) vs (6.7±1.3), P<0.0001], with a larger arterial lumen diameter in choke area(60.14±6.99 vs 28.01±5.07μm, P<0.0001). The expression level of VEGF protein increased (P<0.0001). Conclusions Bletilla polysaccharides can promote the survival of flap, through the regulation of angiogenesis, dilation and VEGF expression in the Chokearea.

Key words: Bletilla striata polysaccharide, , , Choke area, , , Cross-boundary flap, , , Angiogenesis

CLC Number:

R331.32

Yue Qin, Chen Rongli, Xiong Jingxi, Wang Tingyi, Cai Zhiheng, Yi Qiushi, Zeng Xinyi. Effect of Bletilla striata polysaccharides on the choke-area of cross-boundary flap in rats[J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 59-64.

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Effect of Bletilla striata polysaccharides on the choke-area of cross-boundary flap in rats

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