大麻二酚对大鼠多重脑震荡炎症反应的抑制作用

doi:10.13418/j.issn.1001-165x.2024.3.07

Abstract

Abstract: Objective To investigate the mechanism by which cannabidiol (CBD) inhibits neuro-inflammation and protects neural tissue in a rat model of multiple cerebral concussions. Methods Rat models of multiple cerebral concussions (MCC) were established, and Hematoxylin-eosin(HE) and immunohistochemical staining were used to assess changes in neurons and microglial cells after multiple cerebral concussions and the protective effects of CBD. Western blot was performed to measure the expression of microglial cell marker Iba-1 and pro-inflammatory factors IL-1β and TNFα in different brain regions. Results HE staining revealed significant pathological changes in neurons following MCC in rats compared to the Sham group. CBD intervention led to a restoration of normal neuronal morphology, with a more pronounced effect observed in the high-dose group. Immunohistochemical staining showed that microglial cells in the Sham group were in a resting state, while their numbers increased and exhibited an activated state after MCC. CBD intervention resulted in a shift of microglial cells towards a resting state, with a more significant effect observed in the high-dose group. Western blot demonstrated a significant upregulation of Iba-1, IL-1β, and TNFα protein levels in different brain regions after MCC (P<0.05). CBD intervention led to a downregulation of Iba-1, IL-1β, and TNFα expression in these brain regions (P<0.05). Conclusions Cannabidiol could alleviate inflammatory response in the cortex and hippocampus of rats with multiple cerebral concussions and exhibit neuroprotective effects.

Key words: Cannabidiol； , , Multiple cerebral concussions； , , Inflammation

CLC Number:

R651.15

Yang Lifang, Yu Chunze, Zhang Xianjun, Wu Chunyun, Yu Jianyun, Yang Li. Inhibition effects of cannabidiol on inflammatory response following multiple cerebral concussions in rats[J]. Chinese Journal of Clinical Anatomy, 2024, 42(3): 278-283.

References

[1] Koza LA, Pena C, Russell M, et al. Immunocal® limits gliosis in mouse models of repetitive mild-moderate traumatic brain injury[J]. Brain Res, 2023, 1808: 148338. DOI: 10.1016/j.brainres.2023.148338.
[2] Mc Fie S, Abrahams S, Patricios J, Suter J, et al. Inflammatory and apoptotic signalling pathways and concussion severity: a genetic association study[J].J Sports Sci, 2018, 36(19): 2226-2234. DOI: 10.1080/02640414.2018.1448570.
[3] Guo H, Zheng L, Xu H, et al. Neurobiological Links between Stress, Brain Injury, and Disease[J]. Oxid Med Cell Longev, 2022, 2022: 8111022. DOI: 10.1155/2022/8111022.
[4] Sen T, Saha P, Gupta R, et al. Aberrant ER Stress Induced Neuronal-IFNβ Elicits White Matter Injury Due to Microglial Activation and T-Cell Infiltration after TBI[J]. J Neurosci, 2020, 40(2): 424-446. DOI: 10.1523/JNEUROSCI.0718-19.2019.
[5] Nguyen T, Nguyen N, Cochran AG, et al. Repeated closed-head mild traumatic brain injury-induced inflammation is associated with nociceptive sensitization[J]. J Neuroinflammation, 2023, 20(1): 196. DOI: 10.1186/s12974-023-02871-1.
[6] Wang L, Wu X, Yang G, et al. Cannabidiol alleviates the damage to dopaminergic neurons in 1-Methyl-4-Phenyl-1,2,3,6-Tetrahy dropyridine-induced Parkinson's Disease mice via regulating neuronal apoptosis and neuroinflammation[J]. Neuroscience, 2022, 498: 64-72. DOI: 10.1016/j.neuroscience.2022.06.036.
[7] Castillo-Arellano J, Canseco-Alba A, Cutler SJ, León F. The polypharmacological effects of cannabidiol[J]. Molecules, 2023, 28(7): 3271. DOI: 10.3390/molecules28073271.
[8] Zhang H, Zhang Z, Wang Z, et al. Current evidence for pharmacological management of pediatric concussion: a systematic review[J]. Exp Ther Med, 2018, 16(3): 2295-2302. DOI: 10.3892/etm.2018.6474.
[9] 于建云,李娟娟, 韩晓华,等. 脑震荡鼠细胞组织病理学研究[J]. 昆明医学院学报, 2003, (2): 1-5.
[10] 杨雪珂, 张晓丽娜, 钟正，等.天麻素通过Notch信号通路调控激活的BV2小胶质细胞的研究[J].中国临床解剖学杂志, 2023, 41(3): 318-323. DOI:10.13418/j.issn.1001-165x.2023.3.12.
[11]Poulose SM, Bielinski DF, Carey A, et al. Modulation of oxidative stress, inflammation, au-tophagy and expression of Nrf2 in hippocampus and frontal cortex of rats fed with açaí-en-riched diets[J]. Nutr Neurosci, 2017, 20(5): 305-315. DOI: 10.1080/1028415X.2015.1125654.
[12]Hanalioglu D, Hanalioglu S, Arango JI, et al. Didymin suppresses microglia pyroptosis and neuroinflammation through the Asc/Caspase-1/GSDMD pathway following experimenta-l intracerebral hemorrhage[J]. Childs Nerv Syst, 2023, 39(7): 1831-1849. DOI: 10.1007/s00381-023-05960-x.
[13]Unlu MD, Asci H, Yusuf Tepebasi M, et al. The ameliorative effects of cannabidiol on methotrexate-induced neuroinflammation and neuronal apoptosis via inhibiting endoplasmic reticulum and mitochondrial stress[J]. J Biochem Mol Toxicol, 2024, 38(1): e23571. DOI: 10.1002/jbt.23571.
[14]Dogan Unlu M, Uysal D, Karakuyu NF, et al. Investigation of neuroprotective and therapeutic effects of cannabidiol in an acute coronary syndrome model[J]. Neurosci Lett, 2024, 825: 137689. DOI: 10.1016/j.neulet.2024.137689.
[15]Patel V, Abu-Hijleh F, Rigg N, et al. Cannabidiol protects striatal neurons by attenuating endoplasmic reticulum stress[J]. Cannabis Cannabinoid Res, 2023, 8(2): 299-308. DOI: 10.1089/can.2022.0090.
[16]Wang X, Lin C, Jin S, et al. Cannabidiol alleviates neuroinflammation and attenuates neuropathic pain via targeting FKBP5[J]. Brain Behav Immun, 2023, 111: 365-375. DOI: 10.1016/j.bbi.2023.05.008.
[17]Aychman MM, Goldman DL, Kaplan JS. Cannabidiol's neuroprotective properties and potential treatment of traumatic brain injuries[J]. Front Neurol, 2023, 14: 1087011. DOI: 10.3389/fneur.2023.1087011.

Inhibition effects of cannabidiol on inflammatory response following multiple cerebral concussions in rats

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