Three-dimensional reconstruction of micro blood vessels, nerves and lymphatic system in the hepatopancreatic ampulla

doi:10.13418/j.issn.1001-165x.2025.2.08

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Chinese Journal of Clinical Anatomy ›› 2025, Vol. 43 ›› Issue (2) : 157-163. DOI: 10.13418/j.issn.1001-165x.2025.2.08

Three-dimensional reconstruction of micro blood vessels, nerves and lymphatic system in the hepatopancreatic ampulla

Liu Jiannan¹, Zhang Weina¹, Wang Sangui², Liu Yan³, Shi Yulian¹, Liu Jiaxuan¹, Li Xiangtian⁴, Xiao Zhaoming¹, Feng Xiaohui², Huang Wenhua¹, Dai Jingxing¹*, Li Yanbing¹*

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Abstract

Objectives To clarify the branches, distribution, arrangement and quantitative relationship of micro arteries, veins, sympathetic nerves and lymphatic vessels around the hepatopancreatic ampulla. Methods 375 continuous paraffin tissue sections were divided into three groups: those in the first group were stained with hematoxylin and eosin (HE), those in the second group with anti-tyrosine hydroxylase antibody, and those in the third group with anti-lymphatic vessel endothelial receptor-1 (LYVE1) antibody staining. The scanning data of the slides were registered by Photoshop and the scanned data slices were superimposed，divided and 3D reconstructed by using Mimics 21.0. The distribution and anastomosis of arteries, veins, sympathetic nerves, lymphatic vessels, as well as their branches around the hepatopancreatic ampulla were analyzed in the three-dimensional models. The relationships among these four structures were also analyzed. Results The micro arteries and veins in the region of hepatopancreatic ampulla accompanied each other, bending walked around the ampulla and closely adhering to it. Sympathetic nerve tracts were clearly distributed around the hepatopancreatic ampulla and were accompanied by the vascular bundles. Lymphatic vessels and blood vessels were concomitant, surrounding the surface of the hepatopancreatic ampulla and lymph nodes. The structure of the two muscle layers of the hepatopancreatic ampulla was clearly distinguished. Conclusions This study provides accurate anatomical basis for the diagnosis and surgery of extrahepatic biliary tract and gallbladder diseases.

Key words

3D reconstruction / / / Hepatopancreatic ampulla / / / Extrahepatic duct / / / Microanatomy

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Liu Jiannan, Zhang Weina, Wang Sangui, Liu Yan, Shi Yulian, Liu Jiaxuan, Li Xiangtian, Xiao Zhaoming, Feng Xiaohui, Huang Wenhua, Dai Jingxing, Li Yanbing. Three-dimensional reconstruction of micro blood vessels, nerves and lymphatic system in the hepatopancreatic ampulla[J]. Chinese Journal of Clinical Anatomy. 2025, 43(2): 157-163 https://doi.org/10.13418/j.issn.1001-165x.2025.2.08

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