Objective To investigate the effects of recombinant human brain natriuretic peptide (rhBNP) on cardiac remodeling, angiogenesis, and the NF2/Mst1 signaling pathway in rats with acute myocardial infarction. Methods According to random number method, 50 male rats were divided into sham operation group, model group, the low, medium and high dose rhBNP groups (10 rats separately). Acute myocardial infarction models were established in the remaining rats except the sham group. After successful modeling, the rats in the low, medium and high dose rhBNP groups were injected with 5, 10 or 15μg/kg rhBNP solution in the jugular vein, and the other rats were injected with equal volume of normal saline. LVIDd, LVIDs, LVEDV, LVESV were detected by cardiac ultrasound. LVEDP and ±dp/dt max were detected by BL-420 biological function test. BW, THW/BW, LVW/BW were tested. TUNEL was used to detect the apoptosis rate of myocardial cells. Myocardial angiogenesis was detected by immunohistochemistry. The protein expressions of NF2, Mst1, Bax and Bcl-2 were detected by Western blot. Results Compared with the sham group, rats in the model group showed increased LVIDd, LVIDs, LVEDV, LVESV, LVEDP, THW/BW, LVW/BW, myocardial cell apoptosis rate, and increased expression of NF2, Mst1, Bax (P<0.05), while ±dp/dt max and Bcl-2 expression decreased (P<0.05). Compared with the model group, rats in the low, medium, and high-dose rhBNP groups showed decreased LVIDd, LVIDs, LVEDV, LVESV, LVEDP, THW/BW, LVW/BW, cell apoptosis rate, and decreased expression of NF2, Mst1, Bax (P<0.05), while ±dp/dt max and Bcl-2 expression increased (P<0.05). Conclusions rhBNP can significantly improve cardiac remodeling in rats with acute myocardial infarction, reduce myocardial cell apoptosis, and promote angiogenesis in myocardial tissue, which may be related to the activity inhibition of NF2/Mst1 signaling pathway.
Key words
Acute myocardial infarction; /
Recombinant human brain natriuretic peptide; /
Cardiac remodeling; /
/
Angiogenesis; /
/
Neural filament protein; /
/
Mammalian sterile 20-like kinase 1
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