Effect of osteoporosis on proliferation, migration and differentiation of bone marrow mesenchymal stem cells

doi:10.13418/j.issn.1001-165x.2025.2.13

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Chinese Journal of Clinical Anatomy ›› 2025, Vol. 43 ›› Issue (2) : 190-194. DOI: 10.13418/j.issn.1001-165x.2025.2.13

Effect of osteoporosis on proliferation, migration and differentiation of bone marrow mesenchymal stem cells

Shi Yulian, Liu Wenqing, Fan Tingyu, Liu Jiaxuan, Ouyang Jun*, Dai Jingxing*

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Abstract

Objective To study the effect of osteoporosis on the proliferation and differentiation of mouse bone marrow mesenchymal stem cells (mBMSCs), and to provide theoretical and experimental support for the treatment of osteoporosis. Methods Thirty C57BL/6 mice were randomly divided into sham operation group and operation group. Sham operation group only removed the fat around the ovary, and operation group removed the ovary. mBMSCs were taken from the femur and tibia of mice and cultured 8 weeks after operation to observe the changes of proliferation and differentiation. Results The proliferation and migration ability of mBMSCs in osteoporosis mice were decreased. The results of alkaline phosphatase staining showed that mBMSCs had decreased osteogenic differentiation ability in osteoporosis mice. Conclusions The proliferation, migration, and differentiation ability of mBMSCs in osteoporotic mice were decreased.

Key words

Osteoporotic / / / Mouse bone marrow mesenchymal stem cells (mBMSCs) / / / Proliferation / Migrate / / / / Differentiation

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Shi Yulian, Liu Wenqing, Fan Tingyu, Liu Jiaxuan, Ouyang Jun, Dai Jingxing. Effect of osteoporosis on proliferation, migration and differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Clinical Anatomy. 2025, 43(2): 190-194 https://doi.org/10.13418/j.issn.1001-165x.2025.2.13

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