Objective To evaluate the protective effect of bone marrow mesenchymal stem cells (BMSC) on ovarian transplantation after radiotherapy. Methods 40 female Wistar inbred rats were randomly divided into control group and observation group, with 20 rats in each group. BMSC were isolated from 10 rats and cultured and identified. The rats in control group received ovarian transplantation +200 cGy radiation + phosphate buffer solution, and the rats in observation group received ovarian transplantation +200 cGy radiation +BMSC transplantation. The general condition and adverse reactions of rats were observed 1 month after transplantation, and all rats were killed. The serum levels of estradiol (E2), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured by radioimmunoassay. The apoptosis of rat ovarian granulosa cells was detected by in situ end transferase labeling technique. Results The immunophenotype of P3 BMSC showed that the high expression rates of surface markers CD44 (+) and CD90 (+) were 78.04% and 75.17% of cultured cells, respectively. In addition, the expression rates of CD45 (+) and CD34 (+) were relatively low, only 8.05% and 10.40%. After BMSC transplantation, the diet, fur, behavior and mental state of rats in the observation group recovered faster than that in the control group, the body weight increased significantly (P<0.05), and the adverse reaction rate was lower (P=0.028). In addition, compared with the control group, the serum E2 level of rats in the observation group was significantly increased, and the LH and FSH levels were significantly decreased (P<0.001). At the same time, the apoptosis rate of ovarian granulosa cells in the observation group was significantly lower than that in the control group, with statistical significance (P<0.001). Conclusions BMSC can play a protective role after radiotherapy for ovarian transplantation, increase the level of serum E2, decrease the level of LH and FSH, and inhibit the apoptosis of ovarian granulosa cells.
Key words
Bone marrow mesenchymal stem cells /
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Ovarian transplantation /
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Radiotherapy /
Reproductive endocrine hormone /
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Ovarian granulosa cell
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