Objective To investigate the potential mechanism of LINC00626-targeted regulation of the JAK1/STAT3/KHSRP axis in the malignant biological behavior of gastric cancer. Methods qRT-PCR was used to detect the expression levels of LINC00626 and KHSRP mRNA in gastric cancer cell lines (AGS, MGC-803, NCI-87, BGC-823, MNK-45, SGC-7901) and human normal gastric epithelial cell line (GES-1) and 120 cases of gastric cancer tissue specimens and normal tissue specimens. The biological characteristics of cell proliferation, migration and invasion were detected by cell counting kit-8 (CCK-8), scratch and Transwell migration/invasion assays, respectively. The expression levels of JAK/STAT and KHSRP proteins in stably transfected cells were detected by Western blot. The role of LINC00626 in vivo was analyzed in nude mice. Results qRT-PCR results showed that the expression of LINC00626 and KHSRP in gastric cancer cell lines (AGS, MGC-803, NCI-87, BGC-823, MNK-45, SGC-7901) and tumor tissues was significantly higher than that in human normal gastric cancer cells (GES-1) and tissues. Cell function experiments showed that LINC00626 overexpression promoted the proliferation, migration and invasion of gastric cancer cell lines MNK-45 and SGC-7901, and KHSRP was able to restore the ability of proliferation, migration and invasion in gastric cancer cells with knockdown of LINC00626. The results of in vivo animal experiments showed that, compared with the sh-NC group, the tumor volume and weight, cell proliferation rate and the number of lung metastases of gastric cancer in the sh-LINC00626 group were significantly reduced, while the overexpression results were opposite. Signaling pathway experiments showed that compared with the sh-NC group, the expression levels of JAK1 and STAT3 mRNA in the sh-LINC00626 group showed a significant downward trend, while the results in the overexpression group were opposite. Compared with the sh-NC group, the expression of JAK1 and STAT3 in the sh-KHSRP group was significantly down-regulated, and the results in the overexpression group were opposite. Conclusions LINC00626 accelerates the malignant metastasis of gastric cancer by JAK1/STAT3/KHSRP signaling pathway.
Key words
GC; /
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LINC00626; /
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JAK1; /
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STAT3; /
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KHSRP
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