Abstract:

Objective　To investigate the effects of reversine on the proliferation and differentiation of C2C12 myoblasts in vitro.   Methods    C2C12 cells cultured in vitro were treated with 1μM reversine about 12h and 24h respectively, and then flow cytometric analysis was used to detect the apoptosis of treated cells after staining with Annexin-V/PI. Furthermore, myoblast were divided into five groups: control group(CG), reversine group(Revs only), bone induction factor group (BIF only),  reversine and bone induction factor group(Revs and BIF), reversine, bone induction factor and reversine group(Revs and BIF and Revs). The morphological changes of myoblast were observed under the inverted microscope. The mRNA expression of inhibitor of differentiation (ID2), muscle regulatory factor (Myogenin) and myogenic factor (Desmin) was detected by reverse transcription-polymerase chain reaction (RT-PCR) respectively. Results: Compared with group A, significant apoptosis of C2C12 cells appeared in those treated with 1μM reversine about 24h, whereas, no significant apoptosis appeared in group B. Compared with group D, proliferation was significantly inhibited in cells of group E and H, however, cell proliferated and differentiated (fusion) in group F and G. Conclusions    Reversine significantly inhibits the proliferation and differentiation of cultured myoblasts.

Key words: Reversine!C2C12 , myoblast!Apoptosis!Proliferation!Differentiation