基质细胞衍生因子-1α在皮质发育畸形大鼠海马中的表达

Abstract

Abstract:

Objective　To get a message of the expression about stromal cell-derived factor-1α（SDF-1α）in the Malformations of cortical development（MCD）in rats model and investigate the effects in forming abnormal neural circuit in the MCD.　Methods　Sprague-Dawley pregnant rats exposed to X-irradiation on embryonic 16（E16）days can induce MCD in the offspring. We observed the histological changes of the brain with HE staining and Timm staining，and the expression of SDF-1α with immunohistochemistry，RT-PCR and Western-Blot in the pups at postnatal 30 days（P30）and postnatal 60 days（P60）.　Results　①Timm staining demonstrated mossy fiber sprouting was formed in the MCD model；②Immunohistochemical results showed that expression of SDF-1α appeared in the CA1 ectopic neurons of hippocampus；③From the results of RT-PCR and Western-Blot，we got a reduced expression of SDF-1α at P30 and P60 in the model group contrast to control group at the same period. Conclusion the downregulation of SDF-1α may play a role in forming abnormal neural circuit in hippocampus of MCD.

Key words: Malformations of cortical development, Stromal cell-derived factor-1α, , Mossy fiber sprouting, Epilepsy

CLC Number:

R742.1

ZHANG 　Yi, YAN 　Yong, ZHANG Jian-Gang, HUANG 　Min, HE 　Xin. The expression of SDF-1α in the malformation of cortical development model rats[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(1): 72-76.

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The expression of SDF-1α in the malformation of cortical development model rats

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