A novel kind of spinal fusion model-rats interbody fusion

ZHANG Zhao-Fei, JIANG Jian-Ming, FU Zhao-Zong, WANG Feng-Long, WANG  Fei, YANG De-Hong

Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (1) : 83-86.

Chinese Journal of Clinical Anatomy ›› 2011, Vol. 29 ›› Issue (1) : 83-86.

A novel kind of spinal fusion model-rats interbody fusion

  • ZHANG Zhao-fei, JIANG Jian-ming, FU Zhao-zong, WANG Feng-long, WANG Fei, YANG De-hong
Author information +
History +

Abstract

Objective To establish a novel kind of fusion model, and provide the influence of fusion by different bone mass in rats interbody fusion model.    Methods    Sixty male Sprague-Dawley rats underwent interbody fusion with bilateral iliac bone and 1-tailbone autograft into intertailbone. 0.3 cm3 autograft bone was transplanted into the fusion segments of group A and 0.2 cm3 autograft bone was transplanted into that of group B. In contrast,control group C had no bone placed. Fusion segments were removed and analyzed with manual bending and radiography at 3 and 6 weeks, respectively. Histologic analysis was performed at 6 weeks. Half were sacrifced at 3 weeks; the others were sacrifced at 6 weeks.    Results    At 3 weeks, 10%(1/10) of group A rats fused, 0%(0/10) of group B rats fused and 0%(0/10) of group C rats fused by manual palpation. At 6 weeks, 40%(4/10) of group A rats fused, 40%(4/10) of group B rats fused and 0%(0/10) of group C rats fused by manual palpation. By radiographic scoring system  ("0"no bone formation,"5"full fusion), the scores of group A,group B and group C were evaluated as(2.567±0.876),(2.467±0.878)and(0.233±0.316)at 3 weeks after operation, in contrast with(3.233±1.218),(3.267±1.235)and(0.467±0.322)at 6 weeks after operation, respectively. From our gross observation, bone formation with interconnected bone trabecula, which existed in the fusion area of the experimental group, was verified by HE and VG staining at 6 weeks. The neogenesis of the osteoblast and the active bone remodeling with the irregular bone cement lines were seen at the higher magnification. However, there were no existence of bone fusion area in the control group, the fibers tissue is distributed instead.    Conclusions   Our research created a new animal model for spine fusion and verified the effect of quantity of bone autograft on fusion.

Key words

 Rats / Animal models / Spinal fusion / Bone transplantation

Cite this article

Download Citations
ZHANG Zhao-Fei, JIANG Jian-Ming, FU Zhao-Zong, WANG Feng-Long, WANG  Fei, YANG De-Hong. A novel kind of spinal fusion model-rats interbody fusion[J]. Chinese Journal of Clinical Anatomy. 2011, 29(1): 83-86

References


[1] Gupta AR, Shah NR, Patel TC, et al. Perioperative and long-term complications of iliac crest bone graft harvesting for spinal surgery: a quantitative review of the literature
[J]. Int Med J, 2001,8(9):63-66.

[2]  Boden SD, Titus L, Hair G, et al. Lumbar spine fusion by local gene therapy with cDNA encoding a novel osteoinductive protein (LMP-1)
[J].Spine,1998,23(23):2486-2492.

[3] Lawrence JP, Ennis F, White AP, et al. Effect of daily parathyroid hormone (1-34) on lumbar fusion in a rat model
[J].Spine, 2006, 6(4):385-390.

[4]  Abe Y, Takahata M, Ito M, et al. Enhancement of graft bone healing by intermittent administration of human parathyroid hormone (1–34) in a rat spinal arthrodesis model
[J].Bone, 2007,41(5):775-785.

[5]  Huang RC, Khan SN, Sandhu HS, et al. Alendronate inhibits spine fusion in a rat model
[J].Spine,2005,30(22):2516-2522.

[6]  Grauer JN, Bomback DA, Lugo R, et al. Posterolateral lumbar fusions in athymic rats:characterization of a model
[J]. Spine,2004,4(3):281-286.

[7]  O'Loughlin PF, Cunningham ME, Bukata SV, et al. Parathyroid hormone (1-34) augments spinal fusion, fusion mass volume, and fusion mass quality in a rabbit spinal fusion model
[J]. Spine,2009,34(2):121-130.

[8] Grauer JN, Patel TC, Erulkar JS, et al. Evaluation of OP-1 as a graft substitute for intertransverse process lumbar fusion
[J]. Spine,2001,26(2):127-133.

[9]  Steinmann JC, Herkowitz N. Pseudarthosis of the spine
[J].Clin Orthop,1992,(284):80-90.

[10]Boden SD, Schimandle JH, Hutton WC. An experimental lumbar intertransverse process spinal fusion model: radiographic, histologic, and biomechanical healing characteristics
[J]. Spine,1995,20(4):412-420.

Accesses

Citation

Detail

Sections
Recommended

/