1型糖尿病大鼠左心室HCN2和HCN4重构

Abstract

Abstract:

Objective　To investigate the expression of HCN2 and HCN4 in the left ventricles of type 1 diabetes rat.　Methods　60 rats were randomly divided into control group (N, n=20) and diabetes group (T1DM, n=40). For T1DM group, streptozotocin was injected into peritoneal cavity of the animals to establish the model of type 1 diabetes. At the 90d after injecting, the expression of HCN2 and HCN4 were detected by immunofluorescent staining, Western-blot and RT-PCR respectively.　Results　Immuno-fluorescent staining presented the discontinuous punctiform or linear expression of HCN2 in cardiomyocytes membrane, but not for HCN4. However, Both HCN2 and HCN4 were up-regulated at protein(western-blot) and RNA (RT-PCR) levels compared to that of controls.　Conclusions　The over expression of HCN2 and HCN4 maybe participate in the electrical remodeling in DM rats and the developing of ventricular arrhythmia.

Key words: HCN2, HCN4, Left ventricle, T1DM, Rat

CLC Number:

R322.1

WANG Qing-Zhi, ZHOU 　Li, WANG Zhi-Yong, GUO Zhi-Kun. The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(3): 321-324.

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The expression of HCN2 and HCN4 in the left ventricle of type 1 diabetes rat

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