应用破骨细胞-颅骨联合培养体系研究先天性成骨不全的骨再建过程

Abstract

Abstract:

Objective　To evaluate the effects of osteoblast (OB) and osteoclast (OC) on the bone remodeling of osteogenesis imperfecta (OI) adopting osteoclast-calvaria co-culture system in vitro on oim/oim (OI) mouse model.　Methods　Wild (WT) and OI mice were used and compared in this study. OC cells were cultured in calvaria (CAL) in vitro for WT (WTCAL-WTOC group) and OI mice (OICAL-OIOC group) respectively. Tartrate-resistant acid phosphatase (TRAP) staining and alkaline phosphatase (ALP) staining were used to identify OCs and OBs respectively. Bone resorption of OCs was assessed by the area percentage of absorption lacunam, which is the rate of OCs number to the whole calvarial surfaces.　Results　At the culturing time of d7, the number of OCs and OC/OB rate of group OICAL-OIOC were significantly lower than that of group WTCAL-WTOC. However, the OCs number normalized to resorption pit number was significantly greater in group OICAl-OIOC compared to that of WTCAl-WTOC group.　Conclusions　The mechanism of the increased OCs function is partially due to the increased bone turnover in OI mice model for the compensation of decreased OBs function.

Key words: Osteoclast, Bone remodeling, Osteogenesis imperfecta, Osteoclast-calvaria co-culture system

CLC Number:

R682.1

ZHANG 　Gao, ZHONG Qiao-Meng, YUAN 　Kai, CHEN Guo-Xian, LI Ji-Han, CHEN Jian-Ting. The remodeling evaluation of osteopsathyrosis congenita adopting osteoclast-calvaria co-culture system in vitro[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(4): 442-445.

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The remodeling evaluation of osteopsathyrosis congenita adopting osteoclast-calvaria co-culture system in vitro

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