Toll样受体2和4在髋关节滑膜巨噬细胞中的表达

Abstract

Abstract:

Objective　To explore the expression of TLR2 and TLR4 in the macrophages of hip synovium. Methods　Hip synovium tissues were collected from 47 cases during hip surgery in our hospital from 2007 to 2010 and divided into three groups. 24 cases of femoral neck fracture were in group A, 18 cases of femoral head necrosis were in group B, and 5 cases of aseptic loosening were in group C. TLR2 and TLR4 were detected in synovial tissues of three groups by immunohistochemistry (sp), and the positive macrophages were observed, counted and analyzed statistically. Results　The histoscore of TLR2 was 0.27±0.33/HP in Group A, 0.31±0.19/HP in Group B and 1.78±0.18/HP in the Group C. The histoscore of TLR4 was 0.69±0.18/HP in Group A, 0.71±0.31/HP in Group B and 2.00±0.39/HP in Group C. The expression of TLR2 and TLR4 in group C was significantly higher than that in group A and group B(P＜0.001). The expression of TLR4 was significantly higher than TLR2 among the three groups(P＜0.001). Conclusions　TLR2 and TLR4 significantly increases in the macrophages of hip synovium around the prosthesis, which may be involved in macrophage-mediated process of aseptic joint loosening.

Key words: Toll-like receptors (TLRs), Hip joint, synovium, Macrophages, Immunohistochemisty

CLC Number:

R687.4

CENG Zhan-Feng, DING Yue, HU Jie, HUANG Jian-Bin, QIN Chu-Jiang, WEN Chu-Ping, Barden Bertram. The expression of Toll-like receptors 2 and 4 in the macrophages of hip synovium[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(5): 557-560.

References

[1] Keener JD, Callaghan JJ, Goetz DD, et al. Twenty-five-year results after Charnley total hip arthroplasty in patients less than fifty years old: a concise follow-up of a previous report
[J]. J Bone Joint Surg Am,2003,85(6):1066-1072.

[2] Hirayama T, Tamaki Y, Takakubo Y, et al. Toll-like receptors and their adaptors are regulated in macrophages after phagocytosis of lipopolysaccharide-coated titanium particles
[J]. J Orthop Res,2011,29(7):984-992.

[3] Lahdeoja T, Pajarinen J, Kouri VP, et al. Toll-like receptors and aseptic loosening of hip endoprosthesis-a potential to respond against danger signals
[J] ? J Orthop Res,2010,28(2):184-190.

[4] Islam AS, Beidelschies MA, Huml A, et al. Titanium particles activate toll-like receptor 4 independently of lipid rafts in RAW264.7 murine macrophages
[J]. J Orthop Res,2011,29(2):211-217.

[5] Elass E, Coddeville B, Guerardel Y, et al. Identification by surface plasmon resonance of the mycobacterial lipomannan and lipoarabinomannan domains involved in binding to CD14 and LPS-binding protein
[J]. FEBS Lett,2007,581(7):1383-1390.

[6] 丁悦，秦础强，马若凡，等. 磨损颗粒刺激巨噬细胞引起人工关节松动的作用机制
[J]. 中华关节外科杂志（电子版）,2008,2(4):50-52.

[7] Mitchell JA, Paul-Clark MJ, Clarke GW, et al. Critical role of toll-like receptors and nucleotide oligomerisation domain in the regulation of health and disease
[J]. J Endocrinol,2007,193(3):323-330.

[8] Erridge C. Endogenous ligands of TLR2 and TLR4: agonists or assistants?
[J]. J Leukoc Biol,2010,87(6):989-999.

[9] Yang XH, Wu QL, Yu XB, et al. Nestin expression in different tumours and its relevance to malignant grade
[J]. J Clin Pathol,2008,61(4):467-473.

[10] Purdue PE, Koulouvaris P, Potter HG, et al. The cellular and molecular biology of periprosthetic osteolysis
[J]. Clin Orthop Relat Res,2007,(454):251-261.

[11] West AP, Koblansky AA, Ghosh S. Recognition and signaling by toll-like receptors
[J]. Annu Rev Cell Dev Biol,2006,22:409-437.

[12] Medzhitov R, Preston-Hurlburt P, Janeway CJ. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity
[J]. Nature,1997,388(6640):394-397.

[13] Beaulieu LM, Freedman JE. The role of inflammation in regulating platelet production and function: Toll-like receptors in platelets and megakaryocytes
[J]. Thromb Res,2010,125(3):205-209.

[14] Kuroki K, Stoker AM, Sims HJ, et al. Expression of Toll-like receptors 2 and 4 in stifle joint synovial tissues of dogs with or without osteoarthritis
[J]. Am J Vet Res,2010,71(7):750-754.

The expression of Toll-like receptors 2 and 4 in the macrophages of hip synovium

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