可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的生物相容性实验研究

Abstract

Abstract:

Objective　To evaluate the biocompatibility of an injectable chitosan (CS) /nano-hydroxyapatite(HA)/collagen (Col) scaffold.　Methods　rBMSCs was mixed with CS/HA/Col solution and subcutaneously injected into Wistar rats. After 24h, 14d and 28d, the animals were sacrificed and subcutaneous tissues at injecting site were removed. HE, MASSON and immunohistochemical stainings were performed for evaluating the features if scaffolds.　Results　The liquid CS/HA/Col (room temperature) would form the gel after injecting into the subcutaneous tissue. The mixed rBMSCs survived well in the CS/HA/Col gel at even 28 days in vivo. The CS/HA/Col/rBMSCs gel induced less inflammatory reaction in the host tissue compared to that of simple CS/HA/Col gel.　Conclusions　Our results suggest that the CS/HA/Col system can be used to load rBMSCs in vitro homogeneously. The CS/HA/Col system can be used in vivo with the minimally invasive manner and feasible biocompatible environment.

Key words: Injectable scaffold, Tissue engineering, Biocompatibility

CLC Number:

R318.08

CHEN Pan, XU Bo, LI Song-Jian, CHEN Zhong, LIU Cheng-Long. The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(6): 686-689.

References

[1] Zhang W, Liao SS, Cui FZ. Hierarchical self-assembly of nano-fibrils in mineralized collagen
[J]. Chemistry of Materials,2003, 15(16):3221–3226.

[2] Huang Z, Feng QL, Yu B, et al. Biomimetic properties of an injectable chitosan/nano -hydroxyapatite/collagen composite
[J].Materials Science and Engineering,2011,31(3):683–687.

[3]Porter JR, Ruckh TT, Popat KC. Bone tissue engineering: a review in bone biomimetics and drug delivery strategies
[J]. Biotechnol Prog, 2009, 25(6): 1539-1560.

[4]Di Martino A, Sittinger M, Risbud MV. Chitosan：a versatile biopolymer for orthopaedic tissue-engineering
[J]. Biomaterials,2005, 26(30):5983-5990.

[5]Hoemann CD, Sun J, Legare A, et al. Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle
[J]. Osteoarthritis Cartilage, 2005, 13(4): 318-329.

[6] Ma GP, Yang DZ, Li QZ, et al. Injectable hydrogels based on chitosan derivative/polyethylene glycol dimethacrylate/N, N-dimethyla crylamide as bone tissue engineering matrix
[J]. Carbohydrate Polymers, 2009,79(3):620–627.

[7] Muzzarelli RAA. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone
[J]. Carbohydrate Polymers, 2009, 76(2):167–182.

[8]Bartholomew A, Sturgeon C, Siatskas M, et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo
[J]. Experimental Hematology, 2002,30(1): 42–48.

[9]Di Nicola M, Carlo-Stella C, Magni M, et al. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellularor nonspecific mitogenic stimuli
[J]. Blood, 2002,99(10):3838–3843.

[10]Djouad F, Plence P, Bony C, et al. Immunosuppressiveeffect of mesenchymal stem cells favors tumor growth in allogeneic animals
[J]. Blood, 2003,102(10):3837-3844.

[11]Gratzner HG. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine:A new reagent for detection of DNA replication
[J]. Science,1982, 218(4571): 474-475.

[12]Sasaki T, Kitagawa K, Omura-Matsuoka E, et al. The phosphodiesterase inhibitor rolipram promotesSurvival of newborn hippocampal neurons after ischemia
[J]. Stroke, 2007, 38(5): 1597-1605.

[13]Zhao LR, Seema S, Duan WM, et al. Brain repair by hematopoietic growth factors in a rat model of stroke
[J]. Stroke, 2007, 38(9): 2584-2591.

[1]	Ma Hongfeng, Ren Qing, Zheng Mo, Cheng Wanmin, Lian Mei. Application of adipose-derived stem cell aggregate-extracellular matrix composite patch to repair full-thickness skin trauma in external ear canal of New Zealand rabbits [J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 704-709.
[2]	Yang Cheng , Li Zhen, Liao Jianwen, Fan Shicai, Yang Jian, Bai Xiaochun. Research on the biocompatibility and biomechanics of citrate-based biodegradable screws [J]. Chinese Journal of Clinical Anatomy, 2021, 39(2): 182-186.
[3]	WEN Feng, SONG Chen, XIONG Wei-rong, WANG Guo-bao, WANG Xiao-xian, QIU Xiao-zhong, WANG Le-yu. Study on the biosynthesized silver nanoparticles using extractive orange juice and their biocompatibility [J]. Chinese Journal of Clinical Anatomy, 2020, 38(3): 270-276.
[4]	HUANG Shi-jia，WU Wei-le，CHEN Xiao-xing，WANG Le-yu，LIU Cheng-long. Conductive and elastic cryogel for enhancing the proliferation of C2C12 cell [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(3): 277-281.
[5]	WANG Xin-wang,ZHOU Le-bin,MEI Jin,ZHANG De-ming,LIU Dan,DU Ao-ling,CHEN Sheng-hua. Production of decellularized porcine, leporine and murine renal biological scaffold and co-culture exploration of renal scaffolds slices with cells in vitro [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 174-181.
[6]	. Biomechanical evaluation of decellularized porcine bile duct for the construction of bile duct by tissue engineering [J]. Chinese Journal Of Clinical Anatomy, 2015, 33(5): 553-557.
[7]	. Research of lentiviral-mediated Runx2 transferred into bone marrow mesenchymal stem cells promoting osteogenic differentiation [J]. Chinese Journal Of Clinical Anatomy, 2015, 33(3): 311-315.
[8]	ZHANG Wei-Cai, HUANG Ji-Feng, YAN Qiong-Jiao, LI Shi-Pu. PNGF conduit seeded with bone marrow derived mesenchymal stem cells to repair 12-mm sciatic nerve defect of rat [J]. Chinese Journal Of Clinical Anatomy, 2014, 32(3): 325-329.
[9]	YAN Yu-Ting, LI He, YUAN Ru, LI Song-Jian, CHEN Pan. A novel quantitative ultrasound technique for osteogenesis evaluation of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold [J]. Chinese Journal Of Clinical Anatomy, 2014, 32(2): 162-166.
[10]	XIONG Jian-Xi, OU Yang-Jian-An, WANG Da-Beng, RUAN Jian-Meng, SHU Wei-Min, BI Zhi-Jiang, ZHANG Hong. Fabrication of niobium-based biological materials via impregnation and its properties [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(1): 89-94.
[11]	CHEN Kang, WANG Da-Beng, XIONG Jian-Xi, SHU Wei-Min, LIU Jian-Quan, LEI Ming, HU Wen, ZHANG Xiao-Li, LIU Bi. PLLA\β-TCP scaffold guided chondrocyte differentiation of rabbit BMSCs induced by TGF-β1 [J]. Chinese Journal Of Clinical Anatomy, 2012, 30(5): 543-547.
[12]	LV Yu-Meng, CHENG Li-Meng, FEI Guo-Xian, WANG Yu, CUI Fu-Zhai. Characterization and biocompatible evaluation of nHA／RHLC／PLA composite scaffold [J]. Chinese Journal Of Clinical Anatomy, 2011, 29(5): 550-556.
[13]	CUI Xiao-Jun, TAN Yu-Zhen, LI Shao-Hua, GUO Hai-Dong, WANG 　Cun, ZHANG Jian-Kai. The effect of the self-assemble peptide nanofiber scaffold on the proliferation and differentiation of MCSCs in vitro [J]. Chinese Journal Of Clinical Anatomy, 2011, 29(4): 428-432.
[14]	ZHANG 　Kai, WANG Da-Ping, ZHU Wei-Min, LIU Jian-Quan. In vitro biocompatibility of bone marrow mesenchymal stem cells and nano-hydroxyapatite scaffold [J]. Chinese Journal Of Clinical Anatomy, 2011, 29(2): 213-216.

The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Metrics

Comments

Recommended 0