低频电磁场对BDNF基因修饰的BMSCs增殖影响的实验研究

Abstract

Abstract:

Objective　 To explore the effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by Brain-derived neurotrophic factor(BDNF) gene. 　Methods　The replication-deficient recombinant adenoviruses containing BDNF gene（rADBDNF）was structured by the pAd-easy I adenovirus packaging systems in vitro. Rat BMSCs were isolated from rat femoral and tibial bone marrow, cultured and identified. BMSCs infected with rADBDNF were exposed to low frequency electromagnetic fields. The proliferation capacity of BMSCs was analyzed using the trypan blue staining assay and MTT assay, and cell cycles of BMSCs was evaluated by flow cytometry.　Results　BMSCs modified by rADBDNF had no significant change on cell proliferation compared with that of untreated cells (P>0.05). However, electromagnetic field exposed MSCs(LFEMF group) had a significant proliferative improvement compared with the control(P<0.05). The ratio of G2/M cell of the transgenic and LFEMF group was also increased.　Conclusions　LFEMF could enhance the cell proliferation of rat BMSCs modified by BDNF gene.

Key words: Bone mesenchymal stem cells(BMSCs), Cell proliferation, Brain-derived neurotrophic factor(BDNF), Low frequency electromagnetic fields(LFEMF)

CLC Number:

R318.03

QIU Jia-Qi, ZHANG Ming-Sheng, LI Xin-Beng, BAI Wen-Fang, TUN Bo, BAI Li-Meng, XU Wu-Hua. The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(1): 74-78.

References

[1] Daadi MM, Davis AS, Arac A. Human neural stem cell grafts modify microglial response and enhance axonal sprouting in neonatal hypoxic-ischemic brain injury
[J]. Stroke, 2010, 41(3):516-523.

[2] Kawamura K, Kawamura N．Ovarian brain derived neurotrophic factor(BDNF) promotes the development of oocytes into preimplantation embryos
[J].Proc Natl Acad Sci,2005,103(26):9206-9211.

[3] Park DH, Eve DJ, Sanberg PR. Increased neuronal proliferation in the dentate gyrus of aged rats following neural stem cell implantation
[J].　Stem Cells Dev, 2010, 19(2):175-180.

[4] 范萍, 刘永琦, 吴晓晶, 等. 不同条件下骨髓间充质干细胞分离培养及其生物学特性研究
[J].细胞与分子免疫学杂志,2010,26 (4):322-324..

[5] 张鸣生, 何斌, 朱洪翔, 等.不同频率低强度脉冲电磁场对表皮干细胞增殖的影响
[J].中华物理医学与康复杂志,2006,18(11): 726-729.

[6] 张鸣生, 何斌, 朱洪翔, 等.低强度电磁场对创面愈合过程中表皮干细胞增殖和分化的影响
[J]. 中国临床解剖学杂志,2006, 24(6):666-669.

[7] Sun LY,Hsieh DK,Yu TC, et al. Effect of pulsed electromagnetic field on the proliferation and differentiation potential of human bone marrow mesenchymal stem cells
[J]. Bioelectromagnetics, 2009, 30(4):251-260.

[8]De Mattei M, Pellati A,Caruso A, et al. Effects of physical stimulation with electromagnetic field and insulin growth factor-I treatment on proteoglycan synthesis of bovine articular cartilage
[J]. Osteoarthritis Cartilage, 2004, 12(10): 793-800.

[9]Nomura T, Honmou O, Kocsis JD, et al. I.V. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
[J]. Neuroscience, 2005, 136(1):161-169.

[10]许期年,金钧,苗宗宁.脑源性神经生长因子促进骨髓间充质干细胞分化为神经元样细胞的实验研究
[J].苏州大学学报(医学版), 2007, 27(4):520-523.

[11]Schwartz Z,Simon BJ, Boyan BD, et al. Pulsed electromagnetic fields enhance BMP-2 dependent osteoblastic differentiation of human mesenchymal stem cells
[J]. J Orthop Res, 2008, 26(9):1250-1255.

[12] Wei Y, Xiaolin H, Tao S. Effects of extremely low-frequency-pulsed electromagnetic field on different-derived osteoblast-like cells
[J]. Electromagn Biol Med, 2008, 27(3):298-311.

[13] Orendacova J,Racekova E,Galik J, et al. Immunohistochemical study of postnatal neurogenesis after whole-body exposure to electromagnetic fields: evaluation of age- and dose-related changes in rats
[J].Cell Mol Neurobiol, 2009,29 (6-7):981-990.

The effects of low frequency electromagnetic fields on the proliferation of BMSCs modified by BDNF gene

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Metrics

Comments

Recommended 0

[1]	Duan Fangfang, Hou Xiaoxia, Zhou Hanli, Chen Lu, Wang Liuyan, Zhao Xiaoli, Zhang Yujie, Kong Tiandong. Effects of miR-133-3p on the biological behavior of thyroid cancer SW579 cells and the growth of transplanted tumors [J]. Chinese Journal of Clinical Anatomy, 2023, 41(1): 51-57.
[2]	Zhang Haiyan, Sun Lihui, Pan Hongming, Li Lin, Lian Jie, Yu Jing, Lang Weiya . Trichostatin A mediates apoptosis of MDA-MB-231 cells via JNK / MAPK signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2021, 39(2): 174-181.
[3]	LUO Meng-Yang, WU Jiao-Qiong, YANG Bao-Lin, SHU 　Wu, LUO 　Hua, LUO Hua-Gang, CA Wei-Jun. Involvement of nerve innervation on the expression of Ki67 and α-SM-actin in new formed collateral vessels induced by ischemia [J]. Chinese Journal Of Clinical Anatomy, 2010, 28(6): 677-.