离子硅对成骨细胞NF-kappa B核内转录因子活性的影响

Abstract

Abstract:

Objective　To explore the effects of silicon on the activity of nuclear transcription factor NF-kappa B in osteoblasts cultured in vitro.　Methods　MC3T3-E1 osteoablasts were processed by sodium silicate solution (SiO32-) with the final concentration of 1mM and 2 mM for 6h, 12h, 24h and 48h, respectively. Untreated cells were taken as the control groups. Flow cytometry was used to detect the cell cycle and calculate cell proliferation index. Western blotting was used to detect the expressions and changes of NF-kappa B signaling pathway related proteins.　Results　Compared with the controls, significant proliferation of MC3T3-E1 cells was found in the 24h and 48h groups treated with 1 mM silicon. Western blot analysis showed that the pro-proliferation effect of 1 mM silicon on osteoblasts was closely related to the high-expression of p-NF-kappa B.　Conclusions　Trace amounts of silicon released from bone materials can not cause damage of osteoblasts, on contrary, trace amounts of silicate may induce proliferation of osteoblasts by activating NF-kappa B signaling pathway.

Key words: Silicon; , Biomaterial;　Bone repair;　Osteoblast;　NF-kappa B

CLC Number:

R329.2

QIU Xiao-Zhong, WANG Yong-Kuai, WANG Le-Yu, TU Lei, WANG Guo-Bao. Effects of silicon on the activity of the nuclear transcription factor NF-kappa B in osteoblasts[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(1): 84-86.

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Effects of silicon on the activity of the nuclear transcription factor NF-kappa B in osteoblasts

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