Chinese Journal Of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (1): 87-90.

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Differential expression of NPY in superior cervical ganglion of SHR rat

ZHANG Shi1, WEI Shu-ping2, WANG Yi2, XU Peng-xiao2   

  1. 1. Department of Laboratory Animal Centre; 2. Department of Anatomy, Medical College of Chinese People's Armed Police Force, Tianjin 300162, China
  • Received:2011-07-06 Online:2012-01-25 Published:2012-02-03

Abstract:

Objective To explore the effects of neuropeptide Y (NPY) on the occurrence and development of hypertension by observing its expression differentiation in superior cervical ganglion of spontaneous hypertensive rat (SHR). Methods We randomly selected SHR (n=20) and WKY (n=20) and observed the location, the shape, the size and the weight of the superior cervical ganglion. Real-time PCR and immunohistochemical PV-9000 method were used to examine the mRNA and protein expression of NPY in superior cervical ganglion of SHR and WKY. Results:The blood pressure of SHR was obviously higher than that of the WKY group(P<0.05). The results of Real-time PCR and the immunohistochemistry showed that the mRNA and protein expression of NPY in superior cervical ganglion of SHR increased obviously than those in WKY(P<0.05). Conclusions NPY may participate in the occurrence of hypertension by some complex mechanisms.

Key words: SHR, NPY, Cervical ganglion, Real-time PCR

CLC Number: