Abstract:

Objective　To explore how to extract and detect DNA from burned bone.　Methods　30 adult femoral bone were incinerated at 200℃ 1 hour in normal pressure, and then freezed and grinded to be bone meal in the liquid nitrogen before decalcification by ethylenediamine tetraacetic acid(EDTA). The deoxyribonucleic acid (DNA) was extracted by improved phenol-chloroform, and the luminometer was used to detect the concentration and purity of the purified DNA. Subsequently DNA was five-fluorescent labeled, amplified by PCR, followed by capillary electrophoresis and the data analysis.　Results　DNA concentration was (28.5±1.7) ng/μl under the improved phenol-chloroform method, and the 16 genetic locus were detected, with the relative fluorescence units（RFU） of more than 500. The DNA concentration was about  (4.7±0.8) ng /μl, and some gene cound not be detected under the traditional phenol-chloroform extraction.　Conclusions　The DNA extraction and detection can be performed in burned bone by the improved phenol-chloroform method, multiplex polymerase chain reaction, and gene locus analysis.

Key words: DNA detection, Phenol-chloroform, Multiplex polymerase chain reaction, Burned bone