生后不同发育阶段大鼠卵巢内eNOS和iNOS的表达

Abstract

Abstract:

Objective　To observe the expression of eNOS and iNOS in varies and investigate its significance.　Methods　Immunohistochemical technology and image analysis system were utilized to detect the expression of eNOS and iNOS in ovaries from postnatal rat at different developmental days.　Results　Positive reaction for eNOS and iNOS was weak in oocytes of rat ovaries at day 0. In ovaries from older rat than day 4, both of eNOS and iNOS immunoreactivity were intense in health oocytes and very weak in degenerative oocytes. Expression of iNOS was strong positive in follicular cells and theca cells of ovaries from younger rat than day 30，but became very weak in those of growing follicles from older rat than day 30, and increased in those of atretic follicles compared with those in health growing follicles. Positive expression of eNOS was constant. Interstitium and atretic follicles increased in ovaries from older rat than day 240, and interstitium showed an enhanced expression for iNOS. Ovaries from older rat than day 360 became fibrosis, in which iNOS stain was more intensive than eNOS. 　Conclusion　In rat ovaries from different development stages, expression of eNOS is relatively stable and expression of iNOS is the minimum in child-bearing period after sex maturation, but increases gradually with rat age (month) progressing and ovarian functional deterioration.

Key words: Ovary, eNOS, iNOS, Immunohistochemistry, Rat

CLC Number:

Q954.4

JUE Li-Hua, JUE Jiao-Hua, LONG Chi-Feng, LI Mei-Xiang, DAN Jin-Feng, LUO Gong-Mei, MO Zhong-Cheng. Expression of eNOS and iNOS in postnatal rat ovaries at different developmental days[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(2): 203-208.

References

[1] 卜淑敏,夏国良.哺乳动物卵巢中的一氧化氮对卵巢功能的调节作用
[J].生理科学进展，2002，33（3）：273-275.

[2] 朱亮,章孝荣,刘亚，等.一氧化氮与卵母细胞发育
[J].动物医学进展,2010, 31(2):107-110.

[3] 高凤兰，肖秋香，郑莉萍，等.一氧化氮与卵巢生理
[J].生殖医学杂志,2007, 16(2):134-137.

[4] 成志军，章孝荣，陶勇，等.卵母细胞发生阶段猪卵巢一氧化氮合酶的免疫组化定位
[J].中国农业大学学报,2009,14(2):11-16.

[5] Pires PR, Santos NP, Adona PR, et al. Endothelial and inducible nitric oxide synthases in oocytes of cattle
[J]. Anim Reprod Sci, 2009，95(2-4): 156-163.

[6] Tao Y, Fu Z, Zhang M,et al. Immunohistochemical localization of inducible and endothelial nitric oxide synthase in porcine ovaries and effects of NO on antrum formation and oocyte meiotic maturation
[J]. Mol Cell Endocrinol, 2004，222(1-2):93-103.

[7] 邹仲之，李继承.组织学与胚胎学
[M].第七版.北京：人民卫生出版社，2008，190-200.

[8] Tao Y, Xie H, Hong H, et al. Effects of nitric oxide synthase inhibitors on porcin oocyte meiotic maturation
[J]. Zygote, 2005,13(1): 1-9.

[9] 杨善禄，郝依群，王彩华.国人胎儿卵巢组织发生的形态学观察
[J].首都师范大学学报（自然科学版），1993，14（2）：74-78.

[10]罗丽莉，黄菊，傅玉才.内分泌因子、生长因子、细胞因子与卵巢卵泡的发育
[J].中国组织工程研究与临床康复，2007，11（19）：3821-3828。

[11]屈丽华，罗文奇，刘月顺，等.大鼠卵巢的生后发育
[J].四川动物，2009，28（5）：746-748.

[12]Koji T, Teruo Y，Yukari M, et al. Histological observation of the development of follicles and folicular atresia in immature rat ovaries
[J].Acta Med Okayama, 2007,61(5):283-298.

[13]Matsumi H, Yano T, Osuga Y, et al. Regulation of nitric oxide synthase to promote cytostasis in ovarian follicular development
[J].Biol Reprod，2000, 63(1):141-146.

[14]舒建洪，哈利，石玉强，等.卵泡生长发育及其调控的研究进展
[J].西北农林科技大学学报(自然科学版),2004，32（6）：49-53.

[15]田允波，曾书琴，陈学进，等.哺乳动物原始卵泡生长启动的调控
[J].农业生物技术学报，2006,14(4): 618-624.

[16] Ruijin W, Kylie HVH, Natalie KR,et al. Macrophage contributions to ovarian function
[J]. Human Reproduction Update, 2004,10(2 ):119-133.

[17]莫中福，殷秀玲，尹青，等.DahlS高血压大鼠卵巢细胞凋亡相关因素的实验研究
[J].生殖与避孕，2002，22（5）：263-266.

Expression of eNOS and iNOS in postnatal rat ovaries at different developmental days

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Wang Xinghang, Ding Jiayuan, Li Fang, Bao Cuifen, Yan Lijing. Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 33-41.
[2]	Chen Junchen, Cao Wenying, Luo Yanjun, He Wanyi, Zhu Zhenzhen, Peng Zhiqiang. Comparison of the efficacy of aspiration thrombectomy and thrombectomy with combined aspiration and stent retriever in the treatment of anterior circulation cerebral embolism [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 89-93.
[3]	Wang Han, Li Yangyang, Jian Xiaoting, Huang Jingwen, Lan Haiqiang, Hu Jijie, Liao Hua. Loss of CaMKIV signaling aggravates muscle inflammation and affects muscle regeneration [J]. Chinese Journal of Clinical Anatomy, 2023, 41(6): 692-697.
[4]	Kang Li, Cao Yan, Li Hengxi, Li Jiali, Ling Tenghan, Yin Aiping, Zhang Ruilin, Li Ping . Changes of Claudin-5 tight junction protein expression and ameliorative effects of CBD intervention in rats after traumatic brain injury [J]. Chinese Journal of Clinical Anatomy, 2023, 41(6): 698-703.
[5]	Wang Fei, Li Ya’nan, Zhang Xiaolei, Hu Mengni, Li Hanzhang, Ma Jun. Electroacupuncture activates autophagy and improves behavioral responses mechanism in Parkinson's rats brain by regulating PI3K/AKT/mTOR signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2023, 41(6): 709-715.
[6]	Zheng Kai, Luo Xiuling, Zhang Weihao, Liu Yuli, Li Yuming, Liao Shanggao. Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells [J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 543-549.
[7]	Xu Peiqiong, Zheng Zhiguo, Wu Jun. Effect of adiponectin regulating PI3K / AKt signaling pathway on periodontal tissue remodeling during orthodontic tooth movement [J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 583-587.
[8]	Sun Jianyu, Cai Kangxi, Xing Shuai, Xia Yan, Fu Weiwei. Modified latex perfusion technique for visualizing the cerebral vasculature in SD rats [J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 614-618.
[9]	Duan Qifei, Liang Guoyan, Hunag Shuaihao, Chen chong, Ye Yongyu, Chang Yunbing. Research progress on factors influencing clinical efficacy after laminoplasty in patients with degenerative cervical myelopathy [J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 623-626.
[10]	Cao Yingxin, Chi Xiaochen, Bao Cuifen, Li Tingyu, Liu Xia . Study on the protective mechanism of Ginsenoside Rg1 through TNF-α/TNFR1/RIPKs pathway to regulate acute lung injury induced by acute kidney injury [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 409-415.
[11]	Xu Yidan, Wang yanting, Chen shaoFeng, Jing Xing, Xu Dan, Fangfang, Wang Hai, Xie Yun, Zhuang Yuehong. Investigation of Fasudil in its efficacy at improving flap innervation in mice [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 447-452.
[12]	Sun Panwen, Guo Yonglong, Jia Yangyang, Sun Changye, Guo Zhikun. Relationship between calcified tissue of coronary atherosclerosis and c-kit⁺ and sca-1⁺ cells in rats [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 453-458.
[13]	Li Ming, Jia Bingshen, Jiao Tuo, Zhang Ying, Fu Kun. Effect of non-whole time tourniquet application on perioperative outcomes of arthroscopic combined high tibial osteotomy [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 475-480.
[14]	Hua Wei, Li Yanzhu, Lei Tin, Zhou Furong, Liu Yonggan. Treatment strategies for the Altemeier procedure in incarcerated rectal prolapse from a laparoscopic anatomical perspective [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 487-489.
[15]	Song Hehua#, Zhang Qi#, Wu Bihua, Chen Wei, Zhang Tianhua, Nie Kaiyu, Chang Shusen, Deng Chengliang, Li Hai, Zhou Jian, Li Shujun, Wei Zairong. Study on the relationship between the grade IV perforator’s vessels and the survival of reticular blood-supplying flap [J]. Chinese Journal of Clinical Anatomy, 2023, 41(3): 288-295.