维甲酸诱导脊柱裂胎鼠脊髓组织中Caspase-3表达情况

Abstract

Abstract:

Objective To explore caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat.　Methods　Pregnant Wister rats with 10 days were used. Retinoic acid dissolved in olive oil (40mg /ml) were stomach fed for preparing the rat model of spina bifida malformations 135mg / kg). Control group only received olive oil. The animals were divided into 4 groups: pregnancy of 12 days, 15 days, 17 days and 20 days. Immunohistochemical method was used to detect and compare caspase-3 expression in different groups.　Results　The expression of caspase-3 increased at the day 15 after pregnancy, and maintained until day 20 in the spinal tissue of modeled fetal rat, which presented significant difference compared to that of control groups at the same pregnant time. At day 15, day 17 and day 20 of pregnancy, the number of caspase-3 positive cells was more in model animals than the control.　Conclusions　Retinoic acid induced spina bifida fetal rat demonstrates the increased caspase-3 expression in spinal tissue of fetal rats.

Key words: Neural tube defects, Spinal tissue, Apoptosis, Cspase-3

CLC Number:

R722.6

MA Yang-Huan, YUAN Zheng-Wei. Caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(3): 311-314.

References

[1] Bai Y, Chen H, Yuan ZW, et al. Normal and abnormal embryonic development of the anorectum in rats
[J]. J Pediatr Surg，2004，39(4):587-590.

[2] Cecconi F, Piacentini M,Fimia GM. The involvement of cell death and survival in neural tube defects: a distinct role for apoptosis and autophagy
[J]? Cell Death Differ, 2008, 15(7):1170-1177.

[3] Liu XD, Fan RF, Zhang Y, et al. Down-regulation of telomerase activity and activation of caspase-3 are responsible for tanshinone I-induced apoptosis in monocyte leukemia cells in vitro
[J]. Int J Mol Sci, 2010, 11(6):2267-2280.

[4] Au KS, Ashley-Koch A, Northrup H. Epidemiologic and genetic aspects of spina bifida and other neural tube defects
[J]. Dev Disabil Res Rev, 2010, 16(1):6-15.

[5] Chi-FU H, Nami N, Kiyoshi S. The morphogenesis of hindbrain crowding associated with lumbosacral myeloschisis
[J]. Neurol Med Chi (Tokyo), 1989, 96:981-988.

[6] Honarpour N, Du C, Richardson JA, et al .Adult Apaf-1-deficient mice exhibit male infertility
[J] . Dev Biol, 2000, 218(2):248-258.

[7] Kim JY, Huh KH, Park YJ, et al. Molecular mechanisms of cell death of mycophenolic acid-treated primary isolated rat islets: implication of mitogen-activated protein kinase activation
[J]. Transplant Proc, 2008, 40(8):2575-2577.

[8] Dou J, Li X, Cai Y, et al. Human cytomegalovirus induces caspase- dependent apoptosis of megakaryocytic CHRF-288-11 cells by activating the JNKpathway
[J]. Int J Hematol, 2010, 91(4):620-629.

[9] Russell RC, Fang C, Guan KL. An emerging role for TOR?signaling in mammalian tissue and stem cell physiology
[J]. Development, 2011, 138(16):3343-3356.

[10]Platt CC, Nicholls C, Brookes C, et al. Classification of cell signalling in tissue development
[J]. Cell Commun Adhes, 2011, 18(1-2):9-17.

[1]	Chen Ping, Wei Xuemei, Tan Lili. Effect of morroniside on high glucose-induced human retinal vascular endothelial cells oxidative stress and apoptosis by regulating miR-483-5p expression [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 434-439.
[2]	Li Linan, Ding Hanlin, Liu Jinchuan. Effect of miR-181a on the apoptosis of hippocampal neurons induced by sevoflurane by regulating the sirt1 pathway [J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 200-206.
[3]	Miao Yingying, An Leilei, Fu Shengqi. Effects of miRNA-130a-3p on cardiomyocyte injury through regulating NF-κB signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 207-211.
[4]	Duan Fangfang, Hou Xiaoxia, Zhou Hanli, Chen Lu, Wang Liuyan, Zhao Xiaoli, Zhang Yujie, Kong Tiandong. Effects of miR-133-3p on the biological behavior of thyroid cancer SW579 cells and the growth of transplanted tumors [J]. Chinese Journal of Clinical Anatomy, 2023, 41(1): 51-57.
[5]	Gao Shan, Pang Jie, Li Zhanjie, Li Chenxi, Shang Qingyi. Rhizome panacis majoris total saponin inhibits human cytomegalovirus infection of human embryonic lung fibroblasts by up-regulating miR-216a [J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 689-695.
[6]	Zang Chenghao, Yang Jinwei, Ma Wei, Liang Yu, Liu Kuangpin, Liu Wei, Liu Jie, Wang Guodong, Zhang Sijia, Wu Hongjie, Zhu Kewei, Guo Jianhui, Li Liyan. Correlation between intestinal ischemia time and GRP78 expression in rats and its significance [J]. Chinese Journal of Clinical Anatomy, 2022, 40(4): 418-424.
[7]	Wu Yahui, Wang Taofu, Lin Hongqi. The mechanism of dexmedetomidine reducing myocardial ischemia-reperfusion injury and inhibiting apoptosis based on JAK2 / STAT3 signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2022, 40(1): 55-61.
[8]	Ma Daliang, Cui Hongli, Rong Weijiang, Jia Qi, Huang Fuxian. Saikosaponin A attenuates hippocampal neuron damage in rats with cerebral ischemia-reperfusion [J]. Chinese Journal of Clinical Anatomy, 2021, 39(5): 569-574.
[9]	Wu Xuling, Dong Lian, Peng Shuang, Ye Lan, Xu Zucai, Feng Zhanhui. The expression of NHE1 and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy [J]. Chinese Journal of Clinical Anatomy, 2021, 39(4): 431-436.
[10]	Zhang Haiyan, Sun Lihui, Pan Hongming, Li Lin, Lian Jie, Yu Jing, Lang Weiya . Trichostatin A mediates apoptosis of MDA-MB-231 cells via JNK / MAPK signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2021, 39(2): 174-181.
[11]	DENG Yong-hong, ZHANG Li, WANG Jing, YAN Ai-hua . Effects of LncRNA PVT1 on the growth and migration of cervical cancer HeLa cells via targeting miR-190 [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 414-420.
[12]	WU Min, HOU Hui-zhen, SI Ting-lin . Regulatory effects of miR-138 overexpression on proliferation, apoptosis and movement of thyroid carcinoma cell line CAL-62 through inhibiting TCF-4 [J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 425-430.
[13]	LIU Li-xing , MIN Xian-hui , ZHANG Min, HOU Sai-rong. Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms [J]. Chinese Journal of Clinical Anatomy, 2019, 37(3): 261-266.
[14]	ZHANG Zhi-qian, ZHANG Guang-xin, PENG Xiao-dong. The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1 [J]. Chinese Journal of Clinical Anatomy, 2019, 37(3): 292-298.
[15]	ZHAO Yan, HU Jing, YANG Wen-qiang, HE Xin, DU Jia-rui, LI Zong-ze, YANG Jing. The protective effect of carnosine on apoptosis in HBMEC induced by LPS and its mechanism [J]. Chinese Journal Of Clinical Anatomy, 2019, 37(2): 174-178.

Caspase-3 expression in spinal tissue of retinoic acid induced spina bifida fetal rat

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0