Effects of L-NAME on the expression of VE-cadherin in human umbilical vein endothelial cell

YANG Bao-Lin, WU Jiao-Qiong, LUO Meng-Yang, SHU Wu, XIE Feng, LIU Li-Hua, GUAN Ying-Lou, CA Wei-Jun

Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (4) : 412-416.

Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (4) : 412-416.

Effects of L-NAME on the expression of VE-cadherin in human umbilical vein endothelial cell

  • YANG Bao-lin 1,3, WU Xiao-qiong2, LUO Ming-ying1, ZHU Wu1, YE Feng1, LIU Li-hua1, GUAN Ying-lu1, CAI Wei-jun1
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Abstract

Objective    To investigate the effects of N-nitro-L-arginine methyl ester (L-NAME)as a nitric oxide synthase inhibitor on the expression of eNOS and VE-cadherin in human umbilical vein endothelial cell (HUVEC).   Methods   Subculturing HUVECs were identified with specific antibody to CD31. 3 groups (control group, 0.1mmol/L L-NAME group, 1.0 mmol/L L-NAME group) were designed to carry out in this study. The effects of L-NAME on HUVEC activity was assessed with MTT assay. Expression of eNOS and Ve-cadherin protein in HUVEV of all group were detected with immunocytochemistry staining or Western blot. Results   More than 95% subculturing HUVECs were CD31+ cell. MTT assay showed a L-NAME dose-dependent reduction in HUVEC activity when L-NAME was added into medium. The immunofluorescence intensity of eNOS positive staining in 0.1 mmol/L L-NAME group and 1.0mmol/L L-NAME group were obviously decreased as compared with control group(P<0.01). The immunofluorescence intensity and the relative expression of VE-cadherin positive staining in 0.1mmol/L L-NAME group and 1.0 mmol/L L-NAME group were obviously increased as compared with control group(P<0.01).   Conclusions  L-NAME suppresses the expression of eNOS and cell activity, but improves the expression of VE-cadherin in HUVEC.

Key words

N-nitro-L-arginine methyl ester / Human umbilical vein endothelial cell / VE-cadherin

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YANG Bao-Lin, WU Jiao-Qiong, LUO Meng-Yang, SHU Wu, XIE Feng, LIU Li-Hua, GUAN Ying-Lou, CA Wei-Jun. Effects of L-NAME on the expression of VE-cadherin in human umbilical vein endothelial cell[J]. Chinese Journal of Clinical Anatomy. 2012, 30(4): 412-416

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