Effects of L-NAME on the expression of VE-cadherin in human umbilical vein endothelial cell
YANG Bao-Lin, WU Jiao-Qiong, LUO Meng-Yang, SHU Wu, XIE Feng, LIU Li-Hua, GUAN Ying-Lou, CA Wei-Jun
Chinese Journal of Clinical Anatomy ›› 2012, Vol. 30 ›› Issue (4) : 412-416.
Effects of L-NAME on the expression of VE-cadherin in human umbilical vein endothelial cell
Objective To investigate the effects of N-nitro-L-arginine methyl ester (L-NAME)as a nitric oxide synthase inhibitor on the expression of eNOS and VE-cadherin in human umbilical vein endothelial cell (HUVEC). Methods Subculturing HUVECs were identified with specific antibody to CD31. 3 groups (control group, 0.1mmol/L L-NAME group, 1.0 mmol/L L-NAME group) were designed to carry out in this study. The effects of L-NAME on HUVEC activity was assessed with MTT assay. Expression of eNOS and Ve-cadherin protein in HUVEV of all group were detected with immunocytochemistry staining or Western blot. Results More than 95% subculturing HUVECs were CD31+ cell. MTT assay showed a L-NAME dose-dependent reduction in HUVEC activity when L-NAME was added into medium. The immunofluorescence intensity of eNOS positive staining in 0.1 mmol/L L-NAME group and 1.0mmol/L L-NAME group were obviously decreased as compared with control group(P<0.01). The immunofluorescence intensity and the relative expression of VE-cadherin positive staining in 0.1mmol/L L-NAME group and 1.0 mmol/L L-NAME group were obviously increased as compared with control group(P<0.01). Conclusions L-NAME suppresses the expression of eNOS and cell activity, but improves the expression of VE-cadherin in HUVEC.
N-nitro-L-arginine methyl ester / Human umbilical vein endothelial cell / VE-cadherin
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