全反式维甲酸促鼠胚神经干细胞向神经元分化中BMP-2的表达及意义

Abstract

Abstract:

Objective　 To explore the expression and effect of BMP-2 during the rat embryonic stem cells(NSCs) induced by all-trans-retinoic acid（ATRA）differentiating into neurons in vitro.　Methods　 The rat embryonic neural stem cells were differentially induced with 1.0 μmol/L ATRA in vitro. The expression of BMP-2 was respectively investigated by immunocytochemistry, real-time PCR and western blot at the time of inducing 3d, 5d, 7d and 9d, and compared with that of untreated cells in control group.　Results　The highest expression of BMP-2 was at the time of inducing 3d, and then it decreased from inducing 5d following the NSCs differentiating. There was no significant difference between 7d and 9d groups.　Conclusions　The expression of BMP-2 decreases in the progress of NSCS differentiating into neurons in vitro, which indicate that, the inhibition of BMP2 signal pathway participate the differentiation process of NSCs.

Key words: Neural stem cells, All-trans-retinoic acid, BMP-2, Differentiation, Expression

CLC Number:

R338.1

ZHONG De-Jun, LI Sen, KANG Min, XU Shuang, WEI Shu-Yi, WANG Qing, WANG Song. Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(4): 426-430.

References

[1] Martin HM, Thomas GH, David MP, et a1. BMP2 and FGF2 cooperate to induce neural-crest-like fates from fetal and adult CNS stem cells
[J]. J. Cell Sci., 2005,118(24):5849-5860.

[2] Castranio T, Mishina Y. Bmp2 is required for cephalic neural tube closure in the mouse
[J]. Dev Dyn, 2009, 238(1):110-122..

[3] 钟德君, 张德胜, 宋跃明. 全反式维甲酸对鼠胚神经干细胞增殖和分化的作用
[J]. 中国修复重建外科杂志, 2008，22(2):206-211.

[4] 钟德君, 张德胜, 宋跃明. 鼠胚神经干细胞体外培养方法的优化
[J]. 中国组织工程研究与临床康复, 2007,11(50):10088-10092.

[5] Doe CQ. Neural stem cells: balancing self-renewal with differentiation
[J]. Development, 2008, 135(1): 1575 - 1587.

[6] Jacobs S, Lie DC, DeCicco KL, et a1. Retinoic acid is required early during adult neurogenesis in the dentate gyrus
[J]. PNAS, 2006, 103(10): 3902 -3907.

[7] Maden M. Retinoid signalling in the development of the central nervous system
[J]. Nat Rev Neurosci, 2002, 3(11): 843-853.

[8] Miyazono K, Kamiya Y, Morikawa M, et a1. Bone morphogenetic protein receptors and signal transduction
[J]. J. Biochem., 2010，147(1): 35-51.

[9] Aida DG, Margarida S, Stuckey DW, et a1. BMP signalling inhibits premature neural differentiation in the mouse embryo
[J]. Development, 2007, 134(18): 3359-3369.

[10] Marchal L, Luxardi G, Thomé V, et a1. BMP inhibition initiates neural induction via FGF signaling and Zic genes
[J]. PNAS, 2009, 106(41): 17437-17442.

[11] Moustakas A.Smad signalling network
[J]. J. Cell Sci., 2002, 115(17): 3355-3356.

[12] Sakuta H, Takahashi H, Shintani T, et a1. Role of Bone Morphogenic Protein 2 in Retinal Patterning and Retinotectal Projection
[J]. J. Neurosci, 2006, 26(42): 10868 - 10878

[13] Doss MX, Chen S, Winkler J, et a1. Transeriptomie and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells:an insight into mesodermal patterning
[J]. Genome Biol, 2007, 8(9):R184．

[14] Miyazono K. TGF-beta signaling by Smad proteins
[J]. Cytokine Growth Factor Rev, 2000, 11(1.2):15-22.

[15] 张治元, 杨辉, 刘仕勇，等. BMP2在SVZa神经干细胞向神经元分化中的作用
[J].第三军医大学学报, 2003, 25(20):1788-1791.

[16] 钟德君, 康敏, 王清, 等. Wnt-1在全反式维甲酸促鼠胚神经干细胞向神经元分化中的作用
[J]. 中国修复重建外科杂志, 2009,23(6):43-47.

[17] Kasai M, Satoh K, Akiyama T. Wnt signaling regulates the sequential onset of neurogenesis and gliogenesis via induction of BMPs
[J]. Genes Cells, 2005,10(8):777-783.

Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Zeng Xian, Zhou Ying, Yin Yanan, Xie Ting, Li Shuji, Yuan Chunhua. hERG potassium channels mediate the anti-proliferative effects of brucine and strychnine on cancer cells [J]. Chinese Journal of Clinical Anatomy, 2023, 41(6): 684-691.
[2]	LIU Fang, MAO Zhi-rong, DENG Li, CHEN Bo, YUAN Qiong-lan, GAO Xiao-qing. Neuroprotection of GDNF gene-modified NSCs on temporary ischemic stroke in rats [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 408-413.
[3]	LI Ze-yu, WU Zhang , MA Hui-qing, YAO Yu-sheng. Effect and histological analysis of minimally invasive traction of rabbit mandibles with simple screws [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 434-437.
[4]	LI Yong-jin, WU Hui-mei, LIN Yong-peng, HOU Yu,CHEN Bo-lai. HuR induces bone marrow mesenchymal stem cells into chondrogenic differentiation [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(3): 304-308.
[5]	GAO Jun-huai, QIU Su-jun, YIN Jie, SHI Ben-chao, MIN Shao-xiong. Osteogenic differentiation of rat bone marrow mesenchymal stem cells induced by bone morphogenetic protein gene 2 vector [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 187-191.
[6]	SUN Yu, WANG Xiao-bo, WANG Han-qin, YU Jian-xin. The effect of fluid shear stress on expression of Pim-1 in human umbilical vein endothelial cells [J]. Chinese Journal Of Clinical Anatomy, 2017, 35(6): 650-654.
[7]	LAI Gui-hua, HU Xiao-fang, ZHANG Xiang, LU Xing-hao, YU Peng, YU Lei. The expression and cellular distribution of Id2 in myoblast C2C12 under different stimuli [J]. Chinese Journal Of Clinical Anatomy, 2017, 35(2): 172-176.
[8]	LIN Cheng-kai, RONG Li-ming, LIU Bin. Generation of non-integrating induced pluripotent stem cells with episomal plasmids and differentiation into neural stem cells [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(6): 647-654.
[9]	ZHANG Yan-ru,ZHANG Hui, Kaka, ZHANG Ge-chen, SHANG Yan, SUN Hao, HUANG Wen-hua. BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 528-533.
[10]	SONG Hai-yan, ZHANG Yi-min, ZHOU Li, TIAN Yue-min, FU Sheng-qi . Developmental expression of calcium activated chloride ion channels Anoctamin5 in mouse skeletal muscle [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(3): 318-321.
[11]	ZHANG Yan-ling, LIU Jian-jun, XU Hua, YANG Xi-fei. Construction of adeno-associated virus over-expressing vector of complexin-1/2 gene and overexpres -sion of complexin-1/2 on spatial memory impairment in mice of Alzheimer’s disease [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(2): 191-197.
[12]	. Expression of SETD4 in Bac-to-Bac baculovirus expression system [J]. Chinese Journal Of Clinical Anatomy, 2015, 33(4): 426-429.
[13]	. Negative effect of 17β-E2 on lipid droplets through CIDEc and Plin1 in primary human adipocyte [J]. Chinese Journal Of Clinical Anatomy, 2015, 33(3): 320-324.
[14]	TUN Pei-Hua, DAN Jian, CHEN Jian, TAN Cheng. The correlation between non-diabetic cerebral infarction and acute hyperglycemia and its mechanism [J]. Chinese Journal Of Clinical Anatomy, 2015, 33(2): 199-203.
[15]	HUANG Chao, ZHANG Zhi-Jiang, TU Lei, XIE Cai-Jun, CHU Dong-An, BANG Zi-Zhuang, CHEN Cha-Han. MicroRNA-128 regulates differentiation of bone marrow strowal cells induced by Salvia miltiorrhiza [J]. Chinese Journal Of Clinical Anatomy, 2014, 32(4): 427-431.