脊髓压迫性损伤神经纤维溃变和髓鞘碱性蛋白的表达变化

Abstract

Abstract:

Objective　To evaluate the changes of nerve fiber degeneration and myelin basic protein(MBP) expression in rat white matter after the compression spinal cord injury(CSCI). 　Methods　Eighty-four adult Sprague-Dawley rats were randomly divided into 3 groups: normal group（n=12）, operation group（n=36） and the sham group（n=36）. The CSCI model was established with a self-made device for animals of operation group. The operation process in the sham group was the same as the operation group except for the compression of spinal cord. The segments of compressed spinal cord were properly taken at an interval of 1, 3 and 7 day after operation. Luxol fast blue(LFB)， immunofluorescence (IF) staining and western blot were performed to observe the pathological changes of white matters.　Results　LFB staining showed that after compression the degeneration of nerve fiber occurred in the white matter and a great number of fibers were lost. Compared with the normal and sham operation groups, the loss of myelinated fibers was markedly decreased in compressed segments of the model group (P<0.05). IF showed that MBP- positive fibers were degenerated and lost, coinciding with LFB staining. The expression of MBP was progressively significantly declined after compression.　Conclusions　Demyelination occurs, followed by the time-dependently reduced expression of MBP in white matter of model animals. Expression decrease of MBP may be related to the demyelination.

Key words: Spinal cord compression, Nerve degeneration, Demyelination, Myelin basic protein, Rat

CLC Number:

R681.54

WU Xiu-Yu, SUN Shan-Quan, ZHONG Xiao-Yan, ZHANG Bei, HONG Ke-Jian, HUANG Sai-Qin. Alteration of myelin basic protein expression and nerve fiber degeneration after compression injury of rat spinal cord[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(6): 658-662.

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Alteration of myelin basic protein expression and nerve fiber degeneration after compression injury of rat spinal cord

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