皮质发育障碍大鼠海马神经元的体视学方法定量研究

Abstract

Abstract:

Objective　To investigate the variation of number and volume of hippocampus neuron in CA1,CA3 and DG region of rats with cortical dysplasia induced by X-ray using stereological method. Methods 5 normal rats and 5 model rats with 2 months old were used in this study with their brain subsequently and sequentially taken out, embedded in paraffin blocks and being subject to coronal section in thickness of 8 ?滋m. The sections were uniformly and randomly sampled from the consecutive sections of the hippocampus according to the principles of systematic，uniform and random sampling．The sections were stained for Nissl substance for quantification of neuron number in CA1，CA3 ,DG region and volume of the hippocampus using stereological device. Results The number of unilateral hippocampus neurons in CA1，and DG region in normal rats was 8.35×104±1.44×104、8.23×104±1.60×104 and 1.43×105±2.24×104, respectively，and was 3.70×104±1.96×104、3.57×104±1.47×104 and 6.86×104±4.85×104 in model rats with cortical dysplasia, respectively. The volume of the unilateral hippocampus in normal rats and rats with disorders of cortical development was (14.18±1.52) mm3 and (8.49±3.41) mm3, respectively. Compared to normal rats, the number of neurons in CA1,CA3 and DG region and the volume of hippocampus in model rats decreased(P<0.05).　Conclusion　Compared with normal rats, the number and volume of hippocampus neuron in CA1, CA3 and DG regions of rats with cortical dysplasia induced by X-ray is significantly reduced.

Key words: Cortical dysplasia, Epilepsy, Neuron, Hippocampus, Stereology

CLC Number:

R741.02

FENG Fei, CHEN Xiao-Shan, HE Xin, ZHANG Jian-Gang, WEN Meng, TANG Yong, YAN Yong. Quantitative study of variation of number of neurons in cortical dysplasia rat hippocampus by stereological method[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(1): 85-88.

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Quantitative study of variation of number of neurons in cortical dysplasia rat hippocampus by stereological method

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