siRNA沉默TAp73对骨肉瘤细胞的增殖和侵袭力的作用研究

Abstract

Abstract:

Objective　To investigate the pro-apoptosis, anti-migration and anti-proliferation of Saos-2 cells in osteosarcoma through genetic inhibition of expression of TAp73 protein.　Methods　Human osteosarcoma cell line Saos-2 was cultured．A short hairpin RNA targeting TAp73 was constructed and transfected into Saos-2 cell line．The expression of TAp73 protein were examined by Western blot method．The proliferation was assayed by CCK-8．Flow cytometry was used to detect the cell cycle．Transwell test was used to detect the invasion ability of Saos-2.　Results　Western blot revealed notably down-regulated expression at protein levels of Tap 73．The cellular proliferation in the treatment group detected by CCK-8 was active after siRNAtreatment(P<0.05)．The proportion of the cells in the S phase increased．The invasion ability increased from（ 84.17±1.35） to (92.67±2.80 )(P<0.01)．　Conclusions　TAp73 is necessary for human osteosarcoma cell proliferation and invasion．It is probably a potential target for osteosareoma treatment．

Key words: Osteosarcoma, Tap73, SiRNA, Proliferation, Invasion

CLC Number:

R738.1

XUE Jian, JIN An-Min, LV Hai, MIN Shao-Xiong. The influence of down-regulation of TAp73 expression by short hairpin RNA on the proliferation and invasion of osteosarcoma[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(2): 170-173.

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The influence of down-regulation of TAp73 expression by short hairpin RNA on the proliferation and invasion of osteosarcoma

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