同种异体BMSCs、ADSCs移植后的抗原性差异

Abstract

Abstract:

Objective to investigate the antigenic difference of allogeneic bone marrow-derived mesenchymal stem cells( BMSCs) and adipose tissue-derived mesenchymal cells (ADSCs) after their transplantation. Method BMSCs, ADSCs from WKY rats were cultured in vitro and were transplanted into the radial osseous defects in Wistar rats after osteogenic induction; 1、2、4、8 weeks after implantation, tissues from the defective areas was taken for HE staining, IL-2, TGF- β, TNF- α, CD4, CD8 were detected using immunohistochemical staining and quantity of IL-2, TGF- β, TNF- α was determined in supernatant of spleen cell culture using ELISA. Results 1~2 weeks after transplantation of BMSCs, ADSC, mild lymphocyte, leukocyte infiltration can be observed in the defective areas, a small amount of IL-2, TGF- β, TNF- α, CD4, CD8 can be detected; there was no significant difference in the 2 groups. Conclusion No significant difference can be found in BMSCs, ADSCs after transplantation.

Key words: Allogeneic, Mesenchymal stem cells, Antigenicity, Bone defect

CLC Number:

R392.12

LI Lei, QIN Shu-Jian, BAO Cui-Fen, MA Gang, LI Ji, LIU Yu-Zhuo. Antigenic differences of allogeneic BMSCs，ADSCs after transplantation[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(2): 174-179.

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Antigenic differences of allogeneic BMSCs，ADSCs after transplantation

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