Chinese Journal Of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (2): 184-187.

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Brain cell death mechanism induced by high-dose dexamethasone

BAO Cui-fen1,YUAN Jing2, LIANG Jia1, ZHAO Song1   

  1. 1.Centre of Scientific Experiment;2.the First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001,China
  • Received:2012-08-18 Published:2013-04-08

Abstract:

Objective To investigate the brain cell death mechanism induced by high-dose dexamethasone. Methods SD rats were randomly divided into the control group and the experimental group. The experimental group was divided into 7d, 9d, 11d three subgroups. The experimental group received intraperitoneal injection of DX 5mg/kg while the control group received injection with equal volume of physiological saline. light microscopy, electron microscopy, immunohistochemistry and western blot were used to observe the morphological changes of the brain tissue and detect the expression of Active Caspase-3, PARP-1, pULK1 of the brain tissue. Results (1) rat brain tissue structure was clear and normal in the control group. In the 7d experimental group, the brain tissue had no significant pathological changes. Apoptosis and oncosis of the brain cells were observed in the 11d experimental group. (2) In the control group, expression of active Caspase-3, PARP-1, pULK1 was negative. Compared with the control group, expression levels of active Caspase-3, PARP-1,pULK1 were gradually increased with the treatment time in the brain tissue of the experimental group. Conclusion Brain cell death in dexamethasone-induced may be through the activation of Caspase-3 ,PARP-1, pULK1.

Key words: Dexamethasone, Brain cells, Caspase3, (ADP-ribose) polymerase, Phospho-ULK1

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