毛发生长周期对小鼠皮肤创伤愈合的影响

Abstract

Abstract:

Objective　To investigate the skin wound healing speed of C57BL/6 mice at various stages of hair growth cycle.　Methods　Mouse skin wounding models were established and wound healing rate in the following manner was calculated: Wound closure rate (%) = [(Original wound area - Open area on the final day)/Original wound area] ×100%. Wound healing speed of mouse at hair anagen stages and at hair telogen stages was compared. H&E staining was adopted to compare differences in morphological and histological characteristics of wound healing. BrdU was used to detect cell proliferation around wounds.　Results　Wound healing rate of mouse at hair anagen stages was significant higher than that of mouse at telogen stages. H&E staining showed that mice presented relatively more epidermal cell layers around wounds and epidermal cell layers migrated faster at hair anagen stages. BrdU detection showed that mice at hair anagen stages had a larger number of BrdU+ epidermal cells around wounds than mouse at telogen stages.　Conclusion　Mice at hair anagen stages enjoy a faster skin wound healing rate than mice at telogen stages, which provides a basis for further research into the role of hair follicle in wound healing process as well as a guidance on how to choose animal models for study of skin wound healing.

Key words: Wound healing, Hair growth cycle, Animal model

CLC Number:

KONG E-Nan, ZHANG Min, CUI Meng-Qing, LI Liang-Beng, ZHOU Zhi-Chao, ZHANG Lin. Impact of hair growth phase on mouse skin wound healing[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 283-287.

References

[1] 罗满林.实验动物学
[M].北京：中国农业出版社, 2002：87-90.

[2] 张敏,朱晓文,张琳等. C57BL/6小鼠皮肤毛囊发育的研究
[J]. 中国临床解剖学杂志, 2010, 28(1):74-77.

[3] 孙丽, 张敏, 张琳，等. Activin B联合BMSC不同移植方式对大鼠皮肤创伤愈合的治疗
[J]. 中国临床解剖学杂志, 2012, 30(2):197-202.

[4] Müller-R?ver SB, Handjiski C, van der Veen S, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages
[J]. J Invest Dermatol, 2001, 117(1): 3-15.

[5] Zhang M, Liu NY, Wang XE, et al. Activin B promotes epithelial wound healing in vivo through RhoA-JNK signaling pathway
[J]. PLoS One, 2011, 6(9):e25143.

[6] 杨宗成.烧伤治疗学
[M].北京：人民卫生出版社, 2006：180-181.

[7] 邹仲之.组织学与胚胎学
[M]. 北京：人民卫生出版社, 2004：115-116.

[8] Lavker RM, Sun TT, Oshima H, et al. Hair follicle stem cells
[J]. Investig Dermatol Symp Proc, 2003, 8(1):28-38.

[9] Plikus MV, Gay DL, Treffeisen E, et al. Epithelial stem cells and implications for wound repair
[J]. Semin Cell Dev Biol, 2012, 23(9):946-953.

[10] Brownell I, Guevara E, Bai CB, et al. Nerve-derived sonichedgehog defines a niche for hair follicle stem cells capable of becoming epidermal stem cells
[J]. Cell Stem Cell, 2011, 8(5):552-565.

[11] Tiede S, Kloepper JE, Bodò E, et al. Hair follicle stem cells: walking the maze
[J]. Eur J Cell Biol, 2007, 86(7):355-376.

[12] Ito M, Cotsarelis G. Is the hair follicle necessary for normal wound healing
[J]. Invest Dermatol, 2008, 128(5):1059-1061.

[13] Qi SH, Liu P, Xie JL, et al. Experimental study on repairing of nude mice skin defects with composite skin consisting of xenogeneic dermis and epidermal stem cells and hair follicle dermal papilla cells
[J]. Burns, 2008, 34(3):385-392.

[1]	Zhou Yongwei, Zhang Caihua, Li Xiaofei, Cai Xunzi. The repair effect of novel mechanical sensing ion channels Piezo1 and TRPV4 protein siRNA double silencing on osteoarthritis rat model [J]. Chinese Journal of Clinical Anatomy, 2021, 39(6): 692-699.
[2]	MA Chang-rong, LIU Yan, JIANG Ju-quan. Modified rabbit model of right coronary artery ischemia-reperfusion injury [J]. Chinese Journal of Clinical Anatomy, 2020, 38(3): 351-354.
[3]	GAO Hui-min1,2,3, QIU Ming-long2,3, ZHANGg Yan-chao2,3, LIU Hao2,3, MA Si-jie2,3, FU Shan 2,3, LV Yi1,2, YAN Xiao-peng1,2. Establishment of acquired tracheoesophageal fistula by ultra-minimally invasive magnetic compression technique in rabbits [J]. Chinese Journal Of Clinical Anatomy, 2019, 37(2): 223-227.
[4]	ZHONG Zhi-hong, ZHOU Wei, YU Hao-tao, YAN Wei-ming, SHEN Hua-wei, ZHANG Pin. Detection of bone metastasis by small animal micro-PET/CT scan in nude mouse prostate cancer bone metastasis animal models [J]. Chinese Journal Of Clinical Anatomy, 2017, 35(3): 296-300.
[5]	ZHOU Guang-liang, JU Ji-hui, JIANG Guo-dong, ZHANG Yu-jun, LI Lei, HOU Rui-xing. Clinical application of descending genicular artery perforator flap for wounds on extremities [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(6): 693-696.
[6]	ZHANG Zhao-fei, LIU Zhi-xiang, YANG Jun-long, LAI Zhen, JIANG Jian-ming. Evaluation and comparison of different spinal fusion model in rats [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(6): 668-671.
[7]	. Role of iNOS during wound healing of skeletal muscle [J]. Chinese Journal Of Clinical Anatomy, 2015, 33(5): 545-548.
[8]	ZHOU Wei, XU Xi-Chun, JIA Yin-Xing, WANG Ji-You, TU Dong-Jing, HUI Jia-Dong, TU Hao-Chao, DIAO Fei. Developing intervertebral disc degeneration at rabbit lumber spine by using low-temperature plasma radiofrequency ablation [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(6): 696-701.
[9]	SUN Li, WANG Xue-Er, ZHANG Min, CHEN Shi-Xuan, KONG E-Nan, CUI Meng-Qing, ZHANG Lu, ZHANG Lin. The treatment of skin wound healing of rats by different transplantation approaches of BMSCs combination with Activin B [J]. Chinese Journal Of Clinical Anatomy, 2012, 30(2): 197-202.
[10]	DUAN Yong-Chang, TIAN An-Yong, XU Qiao-Lian, ZHENG 　Wei. Anatomy of the extracranial facial nerve of rabbit [J]. Chinese Journal Of Clinical Anatomy, 2011, 29(4): 450-451.
[11]	LIN 　Kai, SHAO Yu-Feng, YANG Xin-Jian, LIU Li-Jun, WANG Da-Beng. Establishment of the rat model of traumatic systemic inflammatory response syndrome [J]. Chinese Journal Of Clinical Anatomy, 2011, 29(4): 446-449.
[12]	ZHANG Zhao-Fei, JIANG Jian-Ming, FU Zhao-Zong, WANG Feng-Long, WANG 　Fei, YANG De-Hong. A novel kind of spinal fusion model-rats interbody fusion [J]. Chinese Journal Of Clinical Anatomy, 2011, 29(1): 83-86.
[13]	LONG Hou-Qing, WEN Chun-Yi, HU 　Yong, LI An-Cheng, LIU Shao-Yu, LI Bi-Bao. Establish of new type rat model of chronic compressive cervical myelopathy [J]. Chinese Journal Of Clinical Anatomy, 2010, 28(5): 566-.
[14]	WANG 　Yu, GAN He-Beng, JIANG Xiao-Jing. A modified rat model of orthotopic liver transplantation model: two-cuff method [J]. Chinese Journal Of Clinical Anatomy, 2010, 28(4): 463-.

Impact of hair growth phase on mouse skin wound healing

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Metrics

Comments

Recommended 0