维甲酸对成纤维细胞及脂肪源干细胞成骨诱导的影响

Abstract

Abstract:

Objective　To explore the effect of retinoic acid(RA) on ALP activity during the osteogenic differentiation of dermal fibroblasts(DFB) and adipose derived stem cells(ADSCs).Method DFB and ADSCs were isolated from suckling mouse aged 5~7 days and cultured in vitro. The DFB were identified by immunofluorescence of vimentin, and the ADSCs were identified by osteogenic differentiation, adipogenic differentiation and cell surface markers detection. Induced by RA and traditional osteogenic liquid separately for 2 weeks, ALP activities were measured by OD value and all specimens were stained by alizarin red.　Results All the specimens were stained by alizarin red, except the control groups. Compared with the control groups, all the induced groups displayed a higher activity of ALP. It is worth noting that ALP expression is higher induced by RA than induced by traditional osteogenic liquid. But the opposite results were obtained in the ADSCs groups. Conclusion　RA is also responsible for osteogenic differentiation of DFB and ADSCs; what is more, the results may be different due to the varieties of cells.

Key words: Fasciology, Retinoic acid, Fibroblasts, Adipose derived stem cells, Osteogenic differentiation

CLC Number:

R338

LIU Yu-Feng, DAI Jing-Xin, YANG Chun, ZHOU Xin, QU Reng-Mei, LI Feng, GOU Ying-Hua, YUAN Lin. Effects of retinoic acid in the osteogenic induction to dermal fibroblasts and adipose derived stem cells[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 303-307.

References

[1] 原林，白宇，黄泳，等. 经络的解剖学发现与筋膜学理论
[J]. 上海针灸杂志,2011,30(1):1-5.

[2] Kawaguchi J, Mee PJ, Smith AG. Osteogenic and chondrogenic differentiation of embryonic stem cells in response to specific growth factors
[J]. Bone,2005,36(5):758-69.

[3] Song HM, Nacamuli RP, Xia W, et al. High-dose retinoic acid modulates rat calvarial osteoblast biology
[J]. J Cell Physiol, 2005, 202(1):255-262.

[4] 赵娟，石艳，孙波，等. 人皮肤成纤维细胞的体外分离、培养及鉴定
[J]. 吉林大学学报(医学版), 2008, 34(5) 899-902.

[5] 戴景兴，杨林林，曲戎梅，等. 差速贴壁法分离培养脂肪源间充质干细胞
[J]. 中国临床解剖学杂志, 2007, 25(3):313-316.

[6] 段富华，杨春，杨会营，等. 维甲酸对脂肪源干细胞中生殖标记表达的影响
[J]. 南方医科大学学报,2012,32(1) :32-36.

[7] Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies
[J]. Tissue Eng,2001,7(2):211-228.

[8] Lorenz K, Sicker M, Schmelzer E, et al. Multilineage differentiation potential of human dermal skin-derived fibroblasts
[J]. Exp Dermatol, 2008, 17(11):925-932.

[9] 王军琳，刘源，金岩，等. 人重组骨形成蛋白-2诱导NIH3T3细胞向成骨细胞表型转化
[J]. 华西口腔医学杂志, 2004, 22(5):420-422.

[10] Grewal NS, Gabbay JS, Ashley RK, et al. BMP-2 does not influence the osteogenic fate of human adipose-derived stem cells
[J]. Plast Reconstr Surg, 2009,123(2 Suppl):158S-165S.

[11] Koussoulakou DS, Margaritis LH, Koussoulakos SL. Antagonists of retinoic acid and BMP4 affect fetal mouse osteogenesis and odontoblast differentiation
[J]. Pathophysiology, 2011, 18(2):103-109.

[12] Wang A, Ding X, Sheng S, et al. Retinoic acid inhibits osteogenic differentiation of rat bone marrow stromal cells
[J]. Biochem Biophys Res Commun, 2008, 375(3):435-439.

[13] Miyamoto S, Cooper L, Watanabe K, et al. Role of retinoic acid-related orphan receptor-alpha in differentiation of human mesenchymal stem cells along with osteoblastic lineage
[J]. Pathobiology, 2010, 77(1):28-37.

[14] 宋维铭，孙广慈，管正玉，等. 维甲酸对成纤维细胞作用的体外观察
[J]. 中华医学美容杂志, 1998, 4(4):190-194.

[15] 李芳芳. 全反式维甲酸对人牙周膜细胞向成骨分化的影响
[J]. 中国现代医学杂志, 2012, 22(7):60-63.

Effects of retinoic acid in the osteogenic induction to dermal fibroblasts and adipose derived stem cells

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Metrics

Comments

Recommended 0

[1]	Huang Xiaolan, Peng Yan, Yang Yuchao, Fan Tingyu, Sun Bing, Asmat Ullah Khan, Dai Jingxing, Ouyang Jun, Zhong Shizhen. Localization and regulation of cytoskeleton associated proteins PDLIM5 in human fibroblasts [J]. Chinese Journal of Clinical Anatomy, 2022, 40(5): 536-540.
[2]	Li Chen, Zhang Yingjie, Sun Yang, Wang Hongxin. Retinoic acid improves lipopolysaccharide-induced vascular inflammation and oxidative stress through TLR4/NF-κB signaling pathway [J]. Chinese Journal of Clinical Anatomy, 2022, 40(3): 303-308.
[3]	CHEN Xiao-feng, ZHANG Min, WU Wei, ZHANG Lin. Regulation of JNK/ERK signal pathway on Activin A mediated the recombination of F-actin of fibroblasts [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 192-196.
[4]	GAO Jun-huai, QIU Su-jun, YIN Jie, SHI Ben-chao, MIN Shao-xiong. Osteogenic differentiation of rat bone marrow mesenchymal stem cells induced by bone morphogenetic protein gene 2 vector [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(2): 187-191.
[5]	ZHONG De-Jun, LI Sen, KANG Min, XU Shuang, WEI Shu-Yi, WANG Qing, WANG Song. Expression and significance of BMP-2 during rat NSCs induced by ATRA differentiating into neurons in vitro [J]. Chinese Journal Of Clinical Anatomy, 2012, 30(4): 426-430.
[6]	LIU Yin-Beng, HUANG Dong, TUN Wei-Chi, HUANG Yong-Jun, HUANG Guo-Yang, LIN Gao. Effects of breviscapine on proliferation and apoptosis of scar fibroblast in vitro [J]. Chinese Journal Of Clinical Anatomy, 2012, 30(2): 218-220.
[7]	LIAN Rui-Jing, CHEN Ti-Jie, HU Ceng-Lu, ZHANG Xiao-Dong. The depressive effects of sTβRⅡ on Smad signal induced by TGF-β1 in neonatal rat cardiac fibroblasts and myofibroblast differentiation [J]. Chinese Journal Of Clinical Anatomy, 2010, 28(2): 184-.